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Author

Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling

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A dynamin-like protein in Arabidopsis thaliana is involved in biogenesis of thylakoid membranes

Cited 46 times inthomson ciCited 47 times inthomson ci
Title
A dynamin-like protein in Arabidopsis thaliana is involved in biogenesis of thylakoid membranes
Author
Park, JMCho, JHKang, SGJang, HJPih, KTPiao, HLCho, Moo JeHwang, I
Keywords
Arabidopsis dynamin-like 1; Chloroplast; Thylakoid membrane biogenesis; Transgenic plants
Issue Date
199802
Publisher
OXFORD UNIV PRESS
Citation
EMBO JOURNAL, v.17, no.4, pp.859 - 867
Abstract
Dynamin, a GTP-binding protein found in rat brain, plays a role in endocytosis. Suborganellar fractionation studies of Arabidopsis leaf tissue revealed that a dynamin-like protein, ADL1, is localized in the thylakoid membranes of chloroplasts. This notion was supported further by lit vivo targeting experiments using an ADL1-green fluorescent fusion protein and immunogold labeling with the anti-ADL1 antibody. Transgenic plants harboring various deletion mutant genes of ADL1 had a yellow leaf phenotype where the cells had very few chloroplasts, In addition, the remaining chloroplasts appeared morphologically not fully developed, The detailed structure of the chloroplasts revealed by electron microscopy showed a greatly reduced amount of thylakoid membranes. Also, the level of thylakoid membrane proteins such as the light-harvesting complex II and CP29 was greatly reduced in these transgenic plants, When we examined the expression of the ADL1 deletion mutant genes, these genes were highly expressed at the transcriptional level, However, the mutant ADL1s were not detectable at the protein level by Western blot analysis, Moreover, the endogenous ADL1 protein level was greatly reduced in these transgenic plants, probably due to a posttranscriptional silencing effect of the transgenes, We propose, therefore, that ADL1 is involved in the biogenesis of thylakoid membranes.
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ISSN
0261-4189
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