BROWSE

Related Researcher

Author

Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling

ITEM VIEW & DOWNLOAD

Soybean Kunitz trypsin inhibitor (SKTI) confers resistance to the brown planthopper (Nilaparvata lugens Stal) in transgenic rice

Cited 78 times inthomson ciCited 79 times inthomson ci
Title
Soybean Kunitz trypsin inhibitor (SKTI) confers resistance to the brown planthopper (Nilaparvata lugens Stal) in transgenic rice
Author
Lee, SILee, SHKoo, JCChun, HJLim, COMun, JHSong, YHCho, Moo Je
Keywords
Brown planthopper;  Insect resistance;  Soybean Kunitz trypsin inhibitor gene;  Transgenic rice plants
Issue Date
1999
Publisher
SPRINGER
Citation
MOLECULAR BREEDING, v.5, no.1, pp.1 - 9
Abstract
Proteinase inhibitors are widely distributed in animals, plants and microorganisms and their roles in plants are associated with defense against pests. The utilization of proteinase inhibitors for crop protection has been actively investigated with a variety of proteinase inhibitors. Soybean Kunitz trypsin inhibitor (SKTI), one of the major seed storage protein, is synthesized for a short period during seed development. To investigate the role of SKTI in a plant's defense system against insect predation, a recombinant plasmid containing the full-length cDNA of SKTI under control of the CaMV 35S promoter was introduced into rice protoplasts by using the PEG direct gene transfer method and a large number of transgenic rice plants were regenerated. The integration, expression, and inheritance of this gene was demonstrated in R1 and R2 generations by Southern, northern, and western analyses. Accumulation levels (0.05-2.5% of soluble proteins) of SKTI protein were detected in R1 and R2 plants. Bioassay with R1 and R2 transgenic plants revealed that transgenic plants are more resistant to destructive insect pest of rice, brown planthopper (Nilaparvata lugens Stal), than the control plants. Thus, introduction of SKTI into rice plants can be used to control insect pests.
URI
http://scholarworks.unist.ac.kr/handle/201301/6364
DOI
http://dx.doi.org/10.1023/A:1009660712382
ISSN
1380-3743
Appears in Collections:
SLS_Journal Papers
Files in This Item:
There are no files associated with this item.

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU