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Cho, Moo Je
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dc.citation.endPage 149 -
dc.citation.number 1 -
dc.citation.startPage 143 -
dc.citation.title PLANTA -
dc.citation.volume 210 -
dc.contributor.author Kim, WY -
dc.contributor.author Kim, CY -
dc.contributor.author Cheong, NE -
dc.contributor.author Choi, YO -
dc.contributor.author Lee, KO -
dc.contributor.author Lee, SH -
dc.contributor.author Park, JB -
dc.contributor.author Nakano, A -
dc.contributor.author Bahk, JD -
dc.contributor.author Cho, Moo Je -
dc.contributor.author Lee, SY -
dc.date.accessioned 2023-12-22T12:09:42Z -
dc.date.available 2023-12-22T12:09:42Z -
dc.date.created 2014-09-22 -
dc.date.issued 1999-11 -
dc.description.abstract By using the mRNA differential display approach to isolate defense signaling genes active at the early stage of fungal infection two cDNA fragments with high sequence homology to rab-specific GDP-dissociation inhibitors (GDIs) were identified in rice (Oryza sativa L.) suspension cells. Using polymerase-chain-reaction products as probes, two full-length cDNA clones were isolated from a cDNA library of fungal-elicitor-treated rice, and designated as OsGDI1 and OsGDI2. The deduced amino acid sequences of the isolated cDNAs exhibited substantial homology to Arabidopsis rab-GDIs. Northern analysis revealed that transcripts detected with the 3'-gene-specific DNA probes accumulated to high levels within 30 min after treatment with a fungal elicitor derived from Magnaporthe grisea. The functionality of the OsGDIs was demonstrated by their ability to rescue the Sec19 mutant of Saccharomyces cerevisiae which is defective in vesicle transport. The proteins, expressed in Escherichia coli, cross-reacted with a polyclonal antibody prepared against bovine rab-GDI. Like bovine rab-GDI, the OsGDI proteins efficiently dissociated rab3A from bovine synaptic membranes. Using the two-hybrid system, it was shown that the OsGDIs specifically interact with the small GTP-binding proteins belonging to the rab subfamily. The specific interaction was also demonstrated in vitro by glutathione S-transferase resin pull-down assay. -
dc.identifier.bibliographicCitation PLANTA, v.210, no.1, pp.143 - 149 -
dc.identifier.doi 10.1007/s004250050663 -
dc.identifier.issn 0032-0935 -
dc.identifier.scopusid 2-s2.0-0039002154 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/6360 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0039002154 -
dc.identifier.wosid 000083866900017 -
dc.language 영어 -
dc.publisher SPRINGER -
dc.title Characterization of two fungal-elicitor-induced rice cDNAs encoding functional homologues of the rab-specific GDP-dissociation inhibitor -
dc.type Article -
dc.description.journalRegisteredClass scopus -

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