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Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling

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Expressed sequence tags of Chinese cabbage flower bud cDNA

Cited 62 times inthomson ciCited 63 times inthomson ci
Title
Expressed sequence tags of Chinese cabbage flower bud cDNA
Author
Lim, COKim, HYKim, MGLee, SIChung, WSPark, SHHwang, IHCho, Moo Je
Keywords
BRASSICA-NAPUS; CAENORHABDITIS-ELEGANS; HUMAN BRAIN; GENES; CLONES
Issue Date
199606
Publisher
AMER SOC PLANT PHYSIOLOGISTS
Citation
PLANT PHYSIOLOGY, v.111, no.2, pp.577 - 588
Abstract
We randomly selected and partially sequenced cDNA clones from a library of Chinese cabbage (Brassica campestris L. ssp. pekinensis) flower bud cDNAs. Out of 1216 expressed sequence tags (ESTs), 904 cDNA clones were unique or nonredundant. Five hundred eighty-eight clones (48.4%) had sequence homology to functionally defined genes at the peptide level. Only 5 clones encoded known flower-specific proteins. Among the cDNAs with no similarity to known protein sequences (628), 184 clones had significant similarity to nucleotide sequences registered in the databases. Among these 184 clones, 142 exhibited similarities at the nucleotide level only with plant ESTs. Also, sequence similarities were evident between these 142 ESTs and their matching ESTs when compared using the deduced amino acid sequences. Therefore, it is possible that the anonymous ESTs encode plant-specific ubiquitous proteins. Our extensive EST analysis of genes expressed in floral organs not only contributes to the understanding of the dynamics of genome expression patterns in floral organs but also adds data to the repertoire of all genomic genes.
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DOI
http://dx.doi.org/10.1104/pp.111.2.577
ISSN
0032-0889
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