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Mitchell, Robert J.
Applied & Environmental Microbiology Lab (AEML)
Research Interests
  • Pathogens, bdellovibrio bacteriovorus, patho-biotechnology

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A biosensor for the detection of gas toxicity using a recombinant bioluminescent bacterium

Cited 56 times inthomson ciCited 53 times inthomson ci
Title
A biosensor for the detection of gas toxicity using a recombinant bioluminescent bacterium
Author
Cheol Gil, G.Mitchell, Robert J.Tai Chang, S.Bock Gu, M.
Keywords
Benzene; Gas toxicity; Recombinant bioluminescent Escherichia coli; Whole cell biosensor
Issue Date
200003
Publisher
ELSEVIER ADVANCED TECHNOLOGY
Citation
BIOSENSORS & BIOELECTRONICS, v.15, no.1-2, pp.23 - 30
Abstract
A whole-cell biosensor was developed for the detection of gas toxicity using a recombinant bioluminescent Escherichia coli harboring a lac::luxCDABE fusion. Immobilization of the cells within LB agar has been done to maintain the activity of the microorganisms and to detect the toxicity of chemicals through the direct contact with gas. Benzene, known as a representative volatile organic compound, was chosen as a sample toxic gas to evaluate the performance of this biosensor based on the bioluminescent response. This biosensor showed a dose-dependent response, and was found to be reproducible. The immobilizing matrices of this biosensor were stored at 4 degrees C and were maintained for at least a month without any noticible change in its activity. The optimal temperature for sensing was 37 degrees C. A small size of this sensor kit has been successfully fabricated, and found to be applicable as a disposable and portable biosensor to monitor the atmospheric environment of a workplace in which high concentrations of toxic gases could be discharged.
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DOI
http://dx.doi.org/10.1016/S0956-5663(99)00074-3
ISSN
0956-5663
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