BROWSE

Related Researcher

Author

Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling

ITEM VIEW & DOWNLOAD

Identification of a calmodulin-regulated soybean Ca2+-ATPase (SCA1) that is located in the plasma membrane

Cited 52 times inthomson ciCited 59 times inthomson ci
Title
Identification of a calmodulin-regulated soybean Ca2+-ATPase (SCA1) that is located in the plasma membrane
Author
Chung, WSLee, SHKim, JCHeo, WDKim, MCPark, Chan YoungPark, HCLim, COKim, WBHarper, JFCho, Moo Je
Keywords
ENDOPLASMIC RETICULUM-TYPE; BINDING DOMAIN; CA2+ PUMP; ARABIDOPSIS-THALIANA; CALCIUM CHANNELS; MESSENGER-RNA; H+-ATPASE; ISOFORMS; PROTEIN; MODULATION
Issue Date
200008
Publisher
AMER SOC PLANT BIOLOGISTS
Citation
PLANT CELL, v.12, no.8, pp.1393 - 1407
Abstract
Ca2+-ATPases are key regulators of Ca2+ ion efflux in all eukaryotes. Animal cells have two distinct families of Ca2+ pumps, with calmodulin-stimulated pumps (type IIB pumps) found exclusively at the plasma membrane. In plants, no equivalent type IIB pump located at the plasma membrane has been identified at the molecular level, although related isoforms have been identified in non-plasma membrane locations. Here, we identify a plant cDNA, designated SCA1 (for soybean Ca2+-ATPase 1), that encodes Ca2+-ATPase and is located at the plasma membrane. The plasma membrane localization was determined by sucrose gradient and aqueous two-phase membrane fractionations and was confirmed by the localization of SCA1p tagged with a green fluorescent protein. The Ca2+-ATPase activity of the SCA1p was increased approximately sixfold by calmodulin (K(1/2) ~ 10 nM). Two calmodulin binding sequences were identified in the N-terminal domain. An N-terminal truncation mutant that deletes sequence through the two calmodulin binding sites was able to complement a yeast mutant (K616) that was deficient in two endogenous Ca2+ pumps. Our results indicate that SCA1p is structurally distinct from the plasma membrane-localized Ca2+ pump in animal cells, belonging instead to a novel family of plant type IIB pumps found in multiple subcellular locations. In plant cells from soybean, expression of this plasma membrane pump was highly and rapidly induced by salt (NaCl) stress and a fungal elicitor but not by osmotic stress.
URI
Go to Link
DOI
http://dx.doi.org/10.1105/tpc.12.8.1393
ISSN
1040-4651
Appears in Collections:
SLS_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU