A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants
Cited 159 times inCited 162 times in
- A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants
- Kim, JC; Lee, SH; Cheong, YH; Yoo, CM; Lee, SI; Chun, HJ; Yun, DJ; Hong, JC; Lee, SY; Lim, CO; Cho, Moo Je
- ABRE motif; BZIP binding enhancement; Cold tolerance; COR gene; SCOF-1; Soybean zinc finger factor
- Issue Date
- PLANT JOURNAL, v.25, no.3, pp.247 - 259
- Cold stress on plants induces changes in the transcription of cold response genes. A cDNA clone encoding C2H2-type zinc finger protein, SCOF-1, was isolated from soybean. The transcription of SCOF-1 is specifically induced by low temperature and abscisic acid (ABA) but not by dehydration or high salinity. Constitutive overexpression of SCOF-1 induced cold-regulated (COR) gene expression and enhanced cold tolerance of non-acclimated transgenic Arabidopsis and tobacco plants. SCOF-1 localized to the nucleus but did not bind directly to either C-repeat/dehydration (CRT/DRE) or ABA responsive element (ABRE), cis-acting DNA regulatory elements present in COR gene promoters. However, SCOF-1 greatly enhanced the DNA binding activity of SGBF-1, a soybean G-box binding bZIP transcription factor, to ABRE in vitro. SCOF-1 also interacted with SGBF-1 in a yeast two-hybrid system. The SGBF-1 transactivated the beta -glucuronidase reporter gene driven by the ABRE element in Arabidopsis leaf protoplasts. Furthermore, the SCOF-1 enhanced ABRE-dependent gene expression mediated by SGBF-1. These results suggest that SCOF-1 may function as a positive regulator of COR gene expression mediated by ABRE via protein-protein interaction, which in turn enhances cold tolerance of plants.
- ; Go to Link
Appears in Collections:
- SLS_Journal Papers
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.