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Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling

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A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants

Cited 159 times inthomson ciCited 162 times inthomson ci
Title
A novel cold-inducible zinc finger protein from soybean, SCOF-1, enhances cold tolerance in transgenic plants
Author
Kim, JCLee, SHCheong, YHYoo, CMLee, SIChun, HJYun, DJHong, JCLee, SYLim, COCho, Moo Je
Keywords
ABRE motif; BZIP binding enhancement; Cold tolerance; COR gene; SCOF-1; Soybean zinc finger factor
Issue Date
2001
Publisher
WILEY-BLACKWELL
Citation
PLANT JOURNAL, v.25, no.3, pp.247 - 259
Abstract
Cold stress on plants induces changes in the transcription of cold response genes. A cDNA clone encoding C2H2-type zinc finger protein, SCOF-1, was isolated from soybean. The transcription of SCOF-1 is specifically induced by low temperature and abscisic acid (ABA) but not by dehydration or high salinity. Constitutive overexpression of SCOF-1 induced cold-regulated (COR) gene expression and enhanced cold tolerance of non-acclimated transgenic Arabidopsis and tobacco plants. SCOF-1 localized to the nucleus but did not bind directly to either C-repeat/dehydration (CRT/DRE) or ABA responsive element (ABRE), cis-acting DNA regulatory elements present in COR gene promoters. However, SCOF-1 greatly enhanced the DNA binding activity of SGBF-1, a soybean G-box binding bZIP transcription factor, to ABRE in vitro. SCOF-1 also interacted with SGBF-1 in a yeast two-hybrid system. The SGBF-1 transactivated the beta -glucuronidase reporter gene driven by the ABRE element in Arabidopsis leaf protoplasts. Furthermore, the SCOF-1 enhanced ABRE-dependent gene expression mediated by SGBF-1. These results suggest that SCOF-1 may function as a positive regulator of COR gene expression mediated by ABRE via protein-protein interaction, which in turn enhances cold tolerance of plants.
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DOI
http://dx.doi.org/10.1046/j.1365-313x.2001.00947.x
ISSN
0960-7412
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