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Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling

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Soybean ascorbate peroxidase suppresses Bax-induced apoptosis in yeast by inhibiting oxygen radical generation

Cited 46 times inthomson ciCited 51 times inthomson ci
Title
Soybean ascorbate peroxidase suppresses Bax-induced apoptosis in yeast by inhibiting oxygen radical generation
Author
Moon, HBaek, DLee, BPrasad, DTLee, SYCho, Moo JeLim, COChoi, MSBahk, JKim, MOHong, JCYun, DJ
Keywords
Apoptosis; Ascorbate peroxidase; Bax-induced cell death; Reactive oxygen species; Saccharomyces cerevisiae
Issue Date
2002
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.290, no.1, pp.457 - 462
Abstract
Bax, a mammalian proapoptotic member of the Bcl-2 family, can induce cell death when expressed in yeast or plant cells. To identify plant Bax inhibitors, we cotransformed a soybean cDNA library and the Bax gene into yeast cells and screened for expressed genes that prevented Bax-induced apoptosis. From the Bax-inhibiting genes isolated, ascorbate peroxidase (sAPX) was selected for characterization. The transcription of sAPX in plants was specifically induced by oxidative stress. Moreover, overexpression of sAPX partially suppressed the H2O2-sensitive phenotype of yeast cytosolic catalase T (Δctt)- and thermosensitive phenotype of cytochrome c peroxidase (Δccp)-deleted mutant cells. Examination of reactive oxygen species (ROS) production using the fluorescence method of dihydrorhodamine 123 oxidation revealed that expression of Bax in yeast cells generated ROS, which was greatly reduced by coexpression with sAPX. Our results collectively suggest that sAPX inhibits the generation of ROS by Bax, which in turn suppresses Bax-induced cell death in yeast.
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DOI
http://dx.doi.org/10.1006/bbrc.2001.6208
ISSN
0006-291X
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