BROWSE

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Author

Cho, Moo Je
Ulsan National Institute of Science and Technology
Research Interests
  • Calcium Signaling

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Characterization of a stamen-specific cDNA encoding a novel plant defensin in Chinese cabbage

Cited 45 times inthomson ciCited 54 times inthomson ci
Title
Characterization of a stamen-specific cDNA encoding a novel plant defensin in Chinese cabbage
Author
Park, HCKang, YHChun, HJKoo, JCCheong, YHKim, CYKim, MCChung, WSKim, JCYoo, JHKoo, YDKoo, SCLim, COLee, SYCho, Moo Je
Keywords
Chinese cabbage (Brassica campestris L. ssp. Pekinensis); Disease resistance; DNA clone; Plant defensin; Small antifungal proteins; Transgenic tobacco plants
Issue Date
2002
Publisher
SPRINGER
Citation
PLANT MOLECULAR BIOLOGY, v.50, no.1, pp.59 - 69
Abstract
We isolated a stamen-specific cDNA, BSD1 (Brassica stamen specific plant defensin 1) that encodes a novel plant defensin peptide in Chinese cabbage (Brassica campestris L. ssp. pekinensis). Plant defensins are antimicrobial peptides containing eight highly conserved cysteine residues linked by disulfide bridges. In BSD1, the eight cysteine residues and a glutamate residue at position 29 are conserved whereas other amino acid residues of the plant defensins consensus sequence are substituted. BSD1 transcripts accumulate specifically in the stamen of developing flowers and its level drops as the flowers mature. The recombinant BSD1 produced in Escherichia coli showed antifungal activity against several phytopathogenic fungi. Furthermore, constitutive over-expression of the BSD1 gene under the control of the cauliflower mosaic virus (CaMV) 35S promoter conferred enhanced tolerance against the Phytophthora parasitica in the transgenic tobacco plants.
URI
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ISSN
0167-4412
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