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Author

Mitchell, Robert J.
Applied & Environmental Microbiology Lab (AEML)
Research Interests
  • Pathogens, bdellovibrio bacteriovorus, patho-biotechnology

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An Escherichia coli biosensor capable of detecting both genotoxic and oxidative damage

Cited 41 times inthomson ciCited 46 times inthomson ci
Title
An Escherichia coli biosensor capable of detecting both genotoxic and oxidative damage
Author
Mitchell, Robert J.Gu,MB
Keywords
GREEN FLUORESCENT PROTEIN; SOS-INDUCING ACTIVITY; BACTERIAL BIOLUMINESCENCE; LUX GENES; DNA; STRESS; CHROMOTEST; AGENTS; BIOLOGY; REGULON
Issue Date
200403
Publisher
SPRINGER
Citation
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, v.64, no.1, pp.46 - 52
Abstract
A two-plasmid dual reporter Escherichia coli biosensor was developed using the genes for bacterial bioluminescence and a mutant of the green fluorescent protein, GFPuv4. To achieve this, the two plasmids, which were derivatives of pBR322 and pACYC184, had compatible origins of replication and different antibiotic selection markers: ampicillin and tetracycline. The parent strains DK1 and ACRG43, each carrying a single plasmid with one of the fusion genes (strain DK1 harboring a fusion of the katG promoter to the lux operon while in ACRG43, the recA promoter was fused with the GFP gene), were responsive to oxidative and DNA damage, respectively, resulting in higher bioluminescence or fluorescence under the relevant toxic conditions. The responses of the dual sensor strain, DUAL22, to various toxicants, e.g., mitomycin C, N-methyl-N-nitro-N-nitrosoguanidine, hydrogen peroxide and cadmium chloride, were characterized and compared with the responses of the parent strains to the same chemicals. Finally, several chemical mixtures that cause various stress responses were tested to demonstrate the ability of this biosensor to detect specific stress responses within a multiple toxicity environment.
URI
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DOI
http://dx.doi.org/10.1007/s00253-003-1418-0
ISSN
0175-7598
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