BROWSE

Related Researcher

Author

Mitchell, Robert J.
Applied & Environmental Microbiology Lab (AEML)
Research Interests
  • Pathogens, bdellovibrio bacteriovorus, patho-biotechnology

ITEM VIEW & DOWNLOAD

Induction of kanamycin resistance gene of plasmid pUCD615 by benzoic acid and phenols

Cited 7 times inthomson ciCited 7 times inthomson ci
Title
Induction of kanamycin resistance gene of plasmid pUCD615 by benzoic acid and phenols
Author
Mitchell, Robert J.Hong, H.N.Gu, M.B.
Keywords
acc (6′)-Ib; Benzoic acid; Bioluminescence; Kanamycin; Uncoupling
Issue Date
200607
Publisher
KOREAN SOC MICROBIOLOGY & BIOTECHNOLOGY
Citation
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY, v.16, no.7, pp.1125 - 1131
Abstract
A kan':: luxCDABE fusion strain that was both highly bioluminescent and responsive to benzoic acid was constructed by transforming E. coli strain W3110 with the plasmid pUCDK, which was constructed by digesting and removing the 7-kb KpnI fragment from the promoterless luxCDABE plasmid pUCD615. Experiments using buffered media showed that this induction was dependent on the pH of the media, which influences the degree of benzoic acid protonation, and the expression levels seen are likely due to acidification of the cytoplasm by uncoupling of benzoic acid. Consequently, the sensitivity of this strain for benzoic acid was increased by nearly 20-fold when the pH was shifted from 8.0 to 6.5. Benzoic acid derivatives and several phenolics also resulted in significantly increased bioluminescent signals. Although these compounds are known to damage membranes and induce the heat-shock response within E coli, bacterial strains harboring mutations in the fadR and rpoH genes, which are responsible for fatly acid biosynthesis during membrane stress and induction of the heat-shock response, respectively, showed that these mutations had no effect on the responses observed.
URI
Go to Link
ISSN
1017-7825
Appears in Collections:
SLS_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU