File Download

There are no files associated with this item.

  • Find it @ UNIST can give you direct access to the published full text of this article. (UNISTARs only)
Related Researcher

MitchellRobertJames

Mitchell, Robert J.
Applied and Environmental Microbiology Lab.
Read More

Views & Downloads

Detailed Information

Cited time in webofscience Cited time in scopus
Metadata Downloads

Chemical-specific continuous biomonitoring using a recombinant bioluminescent bacterium DNT5 (nagR-nagAa :: luxCDABE)

Author(s)
Lee, Jin HyungMitchell, Robert J.Gu, Man Bock
Issued Date
2007-09
DOI
10.1016/j.jbiotec.2007.06.020
URI
https://scholarworks.unist.ac.kr/handle/201301/6254
Fulltext
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34548396389
Citation
JOURNAL OF BIOTECHNOLOGY, v.131, no.3, pp.330 - 334
Abstract
The recombinant bioluminescent bacterium, DNT5, containing a nagR-nagAa::luxCDABE fusion, was tested in a multi-channel continuous monitoring system to evaluate its ability to detect benzoic acid derivatives. Seven chemicals, benzoic acid, salicylic acid, 2,5-dihydroxy benzoic acid, 3,5-dihydroxy benzoic acid, benzene, naphthalene and phenol, were used to characterize the responses of DNT5. This strain responded uniquely to each chemical, and these responses were then evaluated based upon the structures of each chemical. The greatest bioluminescent responses were to salicylic acid and benzoic acid, followed by 2,5-dihydroxy benzoic acid and 3,5-dihydroxy benzoic acid, but DNT5 was unresponsive when exposed to benzene, phenol and naphthalene, suggesting it has a strong preference for benzoic acid derivatives with few or no ring-substituted groups.
Publisher
ELSEVIER SCIENCE BV
ISSN
0168-1656

qrcode

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.