CHEMICAL SYNTHESIS AND CLONING OF HUMAN BETA-ENDORPHIN GENE IN ESCHERICHIA-COLI
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- CHEMICAL SYNTHESIS AND CLONING OF HUMAN BETA-ENDORPHIN GENE IN ESCHERICHIA-COLI
- KIM, MH; LEE, HJ; Min, Kyung-Tai
- Β-endorphin; Escherichia coli; human gene; oligonucleotide synthesis; recombinant DNA
- Issue Date
- HUMANA PRESS INC
- APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.50, no.1, pp.35 - 43
- Total synthesis of human Β-endorphin gene has been designed for the expression in bacterial system. Eight individual oligonucleotides corresponding to the Β-endorphin gene were chemically synthesized and joined through the enzyme-catalyzed reaction. The final yield of the 111-nucleotide-long synthetic Β-endorphin gene construct was about 10% of the total oligonucleotide used. The synthetic human Β-endorphin gene was cloned into the bacterium Escherichia coli, using pUC8 vector and shown to have the correct nucleotide sequences as designed.
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