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Author

Min, Kyung-Tai
Molecular & Cellular Neurobiology Lab(Min Lab)
Research Interests
  • Local protein synthesis, learning & memory, behavior, mitochondrial dynamics

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CHEMICAL SYNTHESIS AND CLONING OF HUMAN BETA-ENDORPHIN GENE IN ESCHERICHIA-COLI

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Title
CHEMICAL SYNTHESIS AND CLONING OF HUMAN BETA-ENDORPHIN GENE IN ESCHERICHIA-COLI
Author
KIM, MHLEE, HJMin, Kyung-Tai
Keywords
Β-endorphin; Escherichia coli; human gene; oligonucleotide synthesis; recombinant DNA
Issue Date
199501
Publisher
HUMANA PRESS INC
Citation
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, v.50, no.1, pp.35 - 43
Abstract
Total synthesis of human Β-endorphin gene has been designed for the expression in bacterial system. Eight individual oligonucleotides corresponding to the Β-endorphin gene were chemically synthesized and joined through the enzyme-catalyzed reaction. The final yield of the 111-nucleotide-long synthetic Β-endorphin gene construct was about 10% of the total oligonucleotide used. The synthetic human Β-endorphin gene was cloned into the bacterium Escherichia coli, using pUC8 vector and shown to have the correct nucleotide sequences as designed.
URI
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DOI
http://dx.doi.org/10.1007/BF02788038
ISSN
0273-2289
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