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Min, Kyung-Tai
Molecular & Cellular Neurobiology Lab(Min Lab)
Research Interests
  • Local protein synthesis, learning & memory, behavior, mitochondrial dynamics

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EcoRI variant N199H has enhanced specific activity

Cited 2 times inthomson ciCited 2 times inthomson ci
Title
EcoRI variant N199H has enhanced specific activity
Author
Kim, JJMin, Kyung-TaiKim, MHAugh, SJKim, BDLee, DS
Keywords
Site-directed mutagenesis
Issue Date
199605
Publisher
ELSEVIER SCIENCE BV
Citation
GENE, v.171, no.1, pp.129 - 130
Abstract
The Asn199 residue of the EcoRI restriction endonuclease has been replaced with other amino acids to investigate whether it mediates nucleotide recognition or catalytic activity. Cassette mutagenesis gave variants of EcoRI: N199D, N199H, N199L, N199R, N199S and N199V. Their relative cleavage rates were found to be in the following order: N199H > EcoRI (wild type; wt) > N199L > N199V > N199S > N199R > N199D. In particular, EcoRI variant N199H showed about a twofold higher specific activity than that of the wt enzyme.
URI
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DOI
http://dx.doi.org/10.1016/0378-1119(96)00003-0
ISSN
0378-1119
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