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DC Field | Value | Language |
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dc.citation.endPage | 1048 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1046 | - |
dc.citation.title | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS | - |
dc.citation.volume | 70 | - |
dc.contributor.author | Kim, Sangwoo | - |
dc.contributor.author | Kim, Yong Hwan | - |
dc.contributor.author | Kim, Kyungji | - |
dc.date.accessioned | 2023-12-22T02:16:45Z | - |
dc.date.available | 2023-12-22T02:16:45Z | - |
dc.date.created | 2014-09-16 | - |
dc.date.issued | 2014-08 | - |
dc.description.abstract | The thermostable d-lactate dehydrogenase from Lactobacillus jensenii (Lj d-LDH) is a key enzyme for the production of the d-form of lactic acid from pyruvate concomitant with the oxidation of NADH to NAD+. The polymers of lactic acid are used as biodegradable bioplastics. The Lj d-LDH protein was crystallized using the hanging-drop vapour-diffusion method in the presence of 28%(w/v) polyethylene glycol 400, 100 mM Tris-HCl pH 9, 200 mM magnesium sulfate at 295 K. X-ray diffraction data were collected to a maximum resolution of 2.1 A. The crystal belonged to space group P3121, with unit-cell parameters a = b = 90.5, c = 157.8 A. With two molecules per asymmetric unit, the crystal volume per unit protein weight (VM) is 2.58 A3 Da-1, which corresponds to a solvent content of approximately 52.3%. The structure was solved by single-wavelength anomalous dispersion using a selenomethionine derivative. | - |
dc.identifier.bibliographicCitation | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, v.70, no.8, pp.1046 - 1048 | - |
dc.identifier.doi | 10.1107/S2053230X14012606 | - |
dc.identifier.issn | 1744-3091 | - |
dc.identifier.scopusid | 2-s2.0-84905444574 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/6016 | - |
dc.identifier.url | http://scripts.iucr.org/cgi-bin/paper?S2053230X14012606 | - |
dc.identifier.wosid | 000340246500008 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Cloning, expression, purification, crystallization and X-ray crystallographic analysis of d-lactate dehydrogenase from Lactobacillus jensenii | - |
dc.type | Article | - |
dc.description.isOpenAccess | FALSE | - |
dc.relation.journalWebOfScienceCategory | Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology; Biophysics; Crystallography | - |
dc.description.journalRegisteredClass | scie | - |
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