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Author

Cho, Yoon-Kyoung
Integrated Nano-Biotech Lab (INBL)
Research Interests
  • Microfluidics, Lab-on-a-chip, personalized biomedical diagnostics, nanobioengineering

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One-step pathogen specific DNA extraction from whole blood on a centrifugal microfluidic device

Cited 141 times inthomson ciCited 135 times inthomson ci
Title
One-step pathogen specific DNA extraction from whole blood on a centrifugal microfluidic device
Author
Cho, Yoon-KyoungLee, Jeong-GunPark, Jong-MyeonLee, Beom-SeokLee, YoungsunKo, Christopher
Keywords
TOTAL ANALYSIS SYSTEMS; CHEMICAL-ANALYSIS SYSTEMS; HEPATITIS-B-VIRUS; REAL-TIME PCR; SAMPLE PREPARATION; COMPACT-DISK; A-CHIP; PLATFORM; ANALYZER; DESIGN
Issue Date
2007
Publisher
ROYAL SOC CHEMISTRY
Citation
LAB ON A CHIP, v.7, no.5, pp.565 - 573
Abstract
We report a fully integrated, pathogen-specific DNA extraction device utilizing centrifugal microfluidics on a polymer based CD platform. By use of the innovative laser irradiated Ferrowax microvalve (LIFM) together with the rapid cell lysis method using laser irradiation on magnetic particles, we could, for the first time, demonstrate a fully integrated pathogen specific DNA extraction from whole blood on a CD. As a model study, DNA extraction experiments from whole blood spiked with Hepatitis B virus (HBV) and E.coli were conducted. The total process of the plasma separation, mixing with magnetic beads conjugated with target specific antibodies, removal of plasma residual, washing and DNA extraction was finished within 12 min with only one manual step, the loading of 100 μL of whole blood. Real-time PCR results showed that the concentration of DNA prepared on a CD using a portable sample preparation device was as good as those by conventional bench top protocol. It demonstrates that our novel centrifugal microfluidics platform enables a full integration of complex biological reactions that require multi-step fluidic control.
URI
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DOI
http://dx.doi.org/10.1039/b616115d
ISSN
1473-0197
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