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Madou, Mark
BIO-MEMS Lab
Research Interests
  • Medical Diagnostics

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Design of a compact disk-like microfluidic platform for enzyme-linked immunosorbent assay

Cited 228 times inthomson ciCited 231 times inthomson ci
Title
Design of a compact disk-like microfluidic platform for enzyme-linked immunosorbent assay
Author
Lai, SWang, SNLuo, JLee, LJYang, STMadou, Mark
Keywords
CAPILLARY ELECTROPHORESIS; MICROCHIP; IMMUNOASSAYS; THEOPHYLLINE
Issue Date
200404
Publisher
AMER CHEMICAL SOC
Citation
ANALYTICAL CHEMISTRY, v.76, no.7, pp.1832 - 1837
Abstract
This paper presents an integrated microfluidic device on a compact disk (CD) that performs an enzyme-linked immunosorbent assay (ELISA) for rat IgG from a hybridoma cell culture. Centrifugal and capillary forces were used to control the flow sequence of different solutions involved in the ELISA process. The microfluidic device was fabricated on a plastic CD. Each step of the ELISA process was carried out automatically by controlling the rotation speed of the CD. The work on analysis of rat IgG from hybridoma culture showed that the microchip-based ELISA has the same detection range as the conventional method on the 96-well microtiter plate but has advantages such as less reagent consumption and shorter assay time over the conventional method.
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DOI
http://dx.doi.org/10.1021/ac0348322
ISSN
0003-2700
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