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Madou, Mark
BIO-MEMS Lab
Research Interests
  • Medical Diagnostics

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Integration of microcolumns and microfluidic fractionators on multitasking centrifugal microfluidic platforms for the analysis of biomolecules

Cited 9 times inthomson ciCited 14 times inthomson ci
Title
Integration of microcolumns and microfluidic fractionators on multitasking centrifugal microfluidic platforms for the analysis of biomolecules
Author
Moschou, EANicholson, ADJia, GYZoval, JVMadou, MarkBachas, LGDaunert, S
Keywords
Biomolecules; Compact disk; Lab-on-a-chip; Microfluidic; Microwave-polymerized monolithic microcolumns
Issue Date
200606
Publisher
SPRINGER HEIDELBERG
Citation
ANALYTICAL AND BIOANALYTICAL CHEMISTRY, v.385, no.3, pp.596 - 605
Abstract
This work demonstrates the development of microfluidic compact discs (CDs) for protein purification and fractionation integrating a series of microfluidic features, such as microreservoirs, microchannels, and microfluidic fractionators. The CDs were fabricated with polydimethylsiloxane (PDMS), and each device contained multiple identical microfluidic patterns. Each pattern employed a microfluidic fractionation feature with operation that was based on the redirection of fluid into an isolation chamber as a result of an overflow. This feature offers the advantage of automated operation without the need for any external manipulation, which is independent of the size and the charge of the fractionated molecules. The performance of the microfluidic fractionator was evaluated by its integration into a protein purification microfluidic architecture. The microfluidic architecture employed a microchamber that accommodated a monolithic microcolumn, the fractionator, and an isolation chamber, which was also utilized for the optical detection of the purified protein. The monolithic microcolumn was polymerized "in situ" on the CD from a monolith precursor solution by microwave-initiated polymerization. This technique enabled the fast, efficient, and simultaneous polymerization of monoliths on disposable CD microfluidic platforms. The design of the CD employed allows the integration of various processes on a single microfluidic device, including protein purification, fractionation, isolation, and detection.
URI
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DOI
http://dx.doi.org/10.1007/s00216-006-0436-z
ISSN
1618-2642
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SLS_Journal Papers

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