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Madou, Mark
BIO-MEMS Lab
Research Interests
  • Medical Diagnostics

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A low-cost, disposable card for rapid polymerase chain reaction

Cited 15 times inthomson ciCited 15 times inthomson ci
Title
A low-cost, disposable card for rapid polymerase chain reaction
Author
Jia, GuangyaoSiegrist, JonathanDeng, ChengwuZoval, Jim V.Stewart, GalePeytavi, RegisHuletsky, AnnBergeron, Michel G.Madou, Mark
Keywords
Disposable card; Ice valve; Miniaturization; Modeling; PCR amplification
Issue Date
200707
Publisher
ELSEVIER SCIENCE BV
Citation
COLLOIDS AND SURFACES B-BIOINTERFACES, v.58, no.1, pp.52 - 60
Abstract
A low-cost, disposable card for rapid polymerase chain reaction (PCR) was developed in this work. Commercially available, adhesive-coated aluminum foils and polypropylene films were laminated with structured polycarbonate films to form microreactors in a card format. Ice valves [1] were employed to seal the reaction chambers during thermal cycling and a Peltier-based thermal cycler was configured for rapid thermal cycling and ice valve actuation. Numerical modeling was conducted to optimize the design of the PCR reactor and investigate the thermal gradient in the reaction chamber in the direction of sample thickness. The PCR reactor was experimentally characterized by using thin foil thermocouples and validated by a successful amplification of 10 copy of E. coli tuf gene in 27 min.
URI
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DOI
http://dx.doi.org/10.1016/j.colsurfb.2007.03.007
ISSN
0927-7765
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