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Madou, Mark
BIO-MEMS Lab
Research Interests
  • Medical Diagnostics

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Gravity force transduced by the MEC-4/MEC-10 DEG/ENaC channel modulates DAF-16/FoxO activity in Caenorhabditis elegans

Cited 11 times inthomson ciCited 11 times inthomson ci
Title
Gravity force transduced by the MEC-4/MEC-10 DEG/ENaC channel modulates DAF-16/FoxO activity in Caenorhabditis elegans
Author
Kim, NahuiDempsey, Catherine M.Kuan, Chih-JenZoval, Jim V.O'Rourke, EyleenRuvkun, GaryMadou, MarkSze, Ji Y.
Keywords
C-ELEGANS; LIFE-SPAN; SIGNALING PATHWAY; NERVOUS-SYSTEM; PROTEIN DAF-16; GENES; NEURONS; LONGEVITY; TOUCH; MECHANOSENSATION
Issue Date
200710
Publisher
GENETICS SOC AM
Citation
GENETICS, v.177, no.2, pp.835 - 845
Abstract
The gravity response is an array of behavioral and physiological plasticity elicited by changes in ambient mechanical force and is an evolutionarily ancient adaptive mechanism. We show in Caenorhabditis elegans that the force of hypergravity is translated into biological signaling via a genetic pathway involving three factors: the degenerin/epithelial Na+ channel (DEG/ENaC) class of mechanosensory channels of touch receptor neurons, the neurotransmitter serotonin, and the FoxO transcription factor DAF-16 known to regulate development, energy metabolism, stress responses, and aging. After worms were exposed to hypergravity for 3 hr, their muscular and neuronal functions were preserved, but they exhibited DAF-16::GFP nuclear accumulation in cells throughout the body and accumulated excess fat. Mutations in MEC-4/MEC-10 DEG/ENaC or its partners MEC-6, MEC-7, and MEC-9 blocked DAF-16::GFP nuclear accumulation induced by hypergravity but did not affect DAF-16 response to other stresses. We show that exogenous serotonin and the antidepressant fluoxetine can attenuate DAF-16::GFP nuclear accumulation in WT animals exposed to hypergravity. These results reveal a novel physiological role of the mechanosensory channel, showing that the perception of mechanical stress controls FoxO signaling pathways and that inactivation of DEG/ENaC may decouple mechanical loading and physiological responses
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DOI
http://dx.doi.org/10.1534/genetics.107.076901
ISSN
0016-6731
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