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DC Field | Value | Language |
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dc.citation.endPage | 3624 | - |
dc.citation.number | 6 | - |
dc.citation.startPage | 3618 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 273 | - |
dc.contributor.author | Kim, MJ | - |
dc.contributor.author | Min, DS | - |
dc.contributor.author | Ryu, SH | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.date.accessioned | 2023-12-22T12:36:27Z | - |
dc.date.available | 2023-12-22T12:36:27Z | - |
dc.date.created | 2014-09-02 | - |
dc.date.issued | 1998-02 | - |
dc.description.abstract | Phospholipase C (PLC)-β4 has been considered to be a mammalian homolog of the NorpA PLC, which is responsible for visual signal transduction in Drosophila. We reported previously the cloning of a cDNA encoding rat phospholipase C-β4 (PLC-β4) (Kim, M.J., Bahk, Y.Y., Min, D. S., Lee, S.J., Ryu, S.H., and Suh, P.-G. (1993) Biochem. Biophys. Res. Commun. 194, 706- 712). We re- port now the isolation and characterization of a splice variant (PLC-β4b). PLC-β4b is identical to the 130-kDa PLC-β4 (PLC-β4a) except that the carboxyl-terminal 162 amino acids of PLC-β4a are replaced by 10 distinct amino acids. The existence of PLC-β4b transcripts in the rat brain was demonstrated by reverse transcription-polymerase chain reaction analysis. Immunological analysis using polyclonal antibody specific for PLC-β4b revealed that this splice variant exists in rat brain cytosol. To investigate functional differences between the two forms of PLC-β4, transient expression studies in COS-7 cells were conducted. We found that PLC-β4a was localized mainly in the particulate fraction of the cell, and it could be activated by Gα(q), whereas PLC-β4b was localized exclusively in the soluble fraction, and it could not be activated by Gα(q). In addition, both PLC-β4a and PLC- β4b were not activated by G-protein βγ-subunits purified from rat brain. These results suggest that PLC-β4b may be regulated by a mechanism different from that of PLC-β4a, and therefore it may play a distinct role in PLC- mediated signal transduction. | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.273, no.6, pp.3618 - 3624 | - |
dc.identifier.doi | 10.1074/jbc.273.6.3618 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.scopusid | 2-s2.0-0032488679 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/5704 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0032488679 | - |
dc.identifier.wosid | 000071822300075 | - |
dc.language | 영어 | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | A cytosolic, G alpha(q)- and beta gamma-insensitive splice variant of phospholipase C-beta 4 | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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