A cytosolic, G alpha(q)- and beta gamma-insensitive splice variant of phospholipase C-beta 4
Cited 29 times inCited 26 times in
- A cytosolic, G alpha(q)- and beta gamma-insensitive splice variant of phospholipase C-beta 4
- Kim, MJ; Min, DS; Ryu, SH; Suh, Pann-Ghill
- PROTEIN ALPHA-SUBUNIT; DROSOPHILA-MELANOGASTER; INOSITOL TRISPHOSPHATE; BOVINE CEREBELLUM; HUMAN PLATELETS; GROWTH-FACTOR; NORPA GENE; C ISOZYME; ACTIVATION; PURIFICATION
- Issue Date
- AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
- JOURNAL OF BIOLOGICAL CHEMISTRY, v.273, no.6, pp.3618 - 3624
- Phospholipase C (PLC)-β4 has been considered to be a mammalian homolog of the NorpA PLC, which is responsible for visual signal transduction in Drosophila. We reported previously the cloning of a cDNA encoding rat phospholipase C-β4 (PLC-β4) (Kim, M.J., Bahk, Y.Y., Min, D. S., Lee, S.J., Ryu, S.H., and Suh, P.-G. (1993) Biochem. Biophys. Res. Commun. 194, 706- 712). We re- port now the isolation and characterization of a splice variant (PLC-β4b). PLC-β4b is identical to the 130-kDa PLC-β4 (PLC-β4a) except that the carboxyl-terminal 162 amino acids of PLC-β4a are replaced by 10 distinct amino acids. The existence of PLC-β4b transcripts in the rat brain was demonstrated by reverse transcription-polymerase chain reaction analysis. Immunological analysis using polyclonal antibody specific for PLC-β4b revealed that this splice variant exists in rat brain cytosol. To investigate functional differences between the two forms of PLC-β4, transient expression studies in COS-7 cells were conducted. We found that PLC-β4a was localized mainly in the particulate fraction of the cell, and it could be activated by Gα(q), whereas PLC-β4b was localized exclusively in the soluble fraction, and it could not be activated by Gα(q). In addition, both PLC-β4a and PLC- β4b were not activated by G-protein βγ-subunits purified from rat brain. These results suggest that PLC-β4b may be regulated by a mechanism different from that of PLC-β4a, and therefore it may play a distinct role in PLC- mediated signal transduction.
- ; Go to Link
Appears in Collections:
- SLS_Journal Papers
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.