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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Novel chemoattractant peptides for human leukocytes

Cited 12 times inthomson ciCited 12 times inthomson ci
Title
Novel chemoattractant peptides for human leukocytes
Author
Bae, YSPark, EYKim, YHe, RYe, RDKwak, JYSuh, Pann-GhillRyu, SH
Keywords
Chemotaxis; Formyl peptide receptor like 1; Peptide; Phagocytes; Phospholipase A2; Superoxide
Issue Date
200311
Publisher
PERGAMON-ELSEVIER SCIENCE LTD
Citation
BIOCHEMICAL PHARMACOLOGY, v.66, no.9, pp.1841 - 1851
Abstract
Phospholipase A2 plays a key role in phagocytic cell functions. By screening a synthetic hexapeptide combinatorial library, we identified 24 novel peptides based on their ability to stimulate arachidonic acid release associated with cytosolic phospholipase A2 activity in differentiated HL60 cells. The identified peptides, that contain the consensus sequence (K/R/M)KYY(P/V/Y)M, also induce intracellular calcium release in a pertussis toxin-sensitive manner showing specific action on phagocytic leukocytes, but not on other cells. Functionally, the peptides stimulate superoxide generation and chemotactic migration in human neutrophils and monocytes. Four of the tested active peptides were ligands for formyl peptide receptor like 1. Among these, two peptides with the consensus sequence (R/M)KYYYM can induce intracellular calcium release in undifferentiated HL60 cells that do not express formyl peptide receptor like 1, indicating usage of other receptor(s). A study of intracellular signaling in differentiated HL60 cells induced by the peptides has revealed that four of the novel peptides can induce extracellular signal-regulated protein kinase activation via shared and distinct signaling pathways, based on their dependence of phospatidylinositol-3-kinase, protein kinase C, and MEK. These peptides provide previously unavailable tools for study of differential signaling in leukocytes.
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DOI
http://dx.doi.org/10.1016/S0006-2952(03)00552-5
ISSN
0006-2952
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