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DC Field | Value | Language |
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dc.citation.endPage | 543 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 537 | - |
dc.citation.title | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.citation.volume | 320 | - |
dc.contributor.author | Irino, Y | - |
dc.contributor.author | Cho, HY | - |
dc.contributor.author | Nakamura, Y | - |
dc.contributor.author | Nakahara, M | - |
dc.contributor.author | Furutani, M | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.contributor.author | Takenawa, T | - |
dc.contributor.author | Fukami, K | - |
dc.date.accessioned | 2023-12-22T10:45:57Z | - |
dc.date.available | 2023-12-22T10:45:57Z | - |
dc.date.created | 2014-09-02 | - |
dc.date.issued | 2004-07 | - |
dc.description.abstract | To date, 12 phospholipase C (PLC) isozymes have been identified in mammals, and they are divided into five classes, β-, γ-, δ-, ε-, and ζ-type. PLCδ-type is reported to be composed of four isozymes, PLCδ1-δ4. Here we report that a screening for mouse PLCδ2 from a BAC library with primers that amplify a specific region of bovine PLCδ2 resulted in isolation of one clone containing the mouse PLCδ4 gene. Furthermore, a database search revealed that there is only one gene corresponding to PLCδ2 and PLCδ4 in the mouse and human genomes, indicating that bovine PLCδ2 is a homologue of human and mouse PLCδ4. However, PLCδ2 Western blot analysis with a widely used commercial anti-PLCδ2 antibody showed an expression pattern distinct from that of PLCδ4 in wild-type mice. In addition, an 80-kDa band, which was recognized by antibody against PLCδ2, was smaller than an 85-kDa band detected by anti-PLCδ4 antibody, and the 80-kDa band was detectable in lysates of brain, testis, and spleen from PLCδ4-deficient mice. We also found that immunoprecipitates from brain lysates with this PLCδ2 antibody contained no PLC activity. From these data, we conclude that bovine PLCδ2 is a homologue of human and mouse PLCδ4, and that three isozymes (δ1, δ3, and δ4) exist in the PLCδ family. | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.320, no.2, pp.537 - 543 | - |
dc.identifier.doi | 10.1016/j.bbrc.2004.05.206 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.scopusid | 2-s2.0-2942744553 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/5678 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=2942744553 | - |
dc.identifier.wosid | 000222567700039 | - |
dc.language | 영어 | - |
dc.publisher | ACADEMIC PRESS INC ELSEVIER SCIENCE | - |
dc.title | Phospholipase C delta-type consists of three isozymes: bovine PLC delta 2 is a homologue of human/mouse PLC delta 4 | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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