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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Phospholipase C delta-type consists of three isozymes: bovine PLC delta 2 is a homologue of human/mouse PLC delta 4

Cited 32 times inthomson ciCited 29 times inthomson ci
Title
Phospholipase C delta-type consists of three isozymes: bovine PLC delta 2 is a homologue of human/mouse PLC delta 4
Author
Irino, YCho, HYNakamura, YNakahara, MFurutani, MSuh, Pann-GhillTakenawa, TFukami, K
Keywords
Mouse genome; Phospholipase C; PLCδ2; PLCδ4
Issue Date
200407
Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
Citation
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, v.320, no.2, pp.537 - 543
Abstract
To date, 12 phospholipase C (PLC) isozymes have been identified in mammals, and they are divided into five classes, β-, γ-, δ-, ε-, and ζ-type. PLCδ-type is reported to be composed of four isozymes, PLCδ1-δ4. Here we report that a screening for mouse PLCδ2 from a BAC library with primers that amplify a specific region of bovine PLCδ2 resulted in isolation of one clone containing the mouse PLCδ4 gene. Furthermore, a database search revealed that there is only one gene corresponding to PLCδ2 and PLCδ4 in the mouse and human genomes, indicating that bovine PLCδ2 is a homologue of human and mouse PLCδ4. However, PLCδ2 Western blot analysis with a widely used commercial anti-PLCδ2 antibody showed an expression pattern distinct from that of PLCδ4 in wild-type mice. In addition, an 80-kDa band, which was recognized by antibody against PLCδ2, was smaller than an 85-kDa band detected by anti-PLCδ4 antibody, and the 80-kDa band was detectable in lysates of brain, testis, and spleen from PLCδ4-deficient mice. We also found that immunoprecipitates from brain lysates with this PLCδ2 antibody contained no PLC activity. From these data, we conclude that bovine PLCδ2 is a homologue of human and mouse PLCδ4, and that three isozymes (δ1, δ3, and δ4) exist in the PLCδ family.
URI
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DOI
http://dx.doi.org/10.1016/j.bbrc.2004.05.206
ISSN
0006-291X
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