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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Phospholipase C-gamma 1 potentiates integrin-dependent cell spreading and migration through Pyk2/paxillin activation

Cited 15 times inthomson ciCited 17 times inthomson ci
Title
Phospholipase C-gamma 1 potentiates integrin-dependent cell spreading and migration through Pyk2/paxillin activation
Author
Choi, Jang HyunYang, Yong-RyoulLee, Seul KiKim, Il-ShinHa, Sang HoonKim, Eung-KyunBae, Yun SooRyu, Sung HoSuh, Pann-Ghill
Keywords
Adhesion; Integrin; Migration; Paxillin; Phospholipase C-γ1; Pyk2; Rac1
Issue Date
200708
Publisher
ELSEVIER SCIENCE INC
Citation
CELLULAR SIGNALLING, v.19, no.8, pp.1784 - 1796
Abstract
Phospholipase C-γ1 (PLC-γ1), which generates two second messengers, namely, inositol-1, 4, 5-trisphosphate and diacylglycerol, is implicated in growth factor-mediated chemotaxis. However, the exact role of PLC-γ1 in integrin-mediated cell adhesion and migration remains poorly understood. In this study, we demonstrate that PLC-γ1 is required for actin cytoskeletal organization and cell motility through the regulation of Pyk2 and paxillin activation. After fibronectin stimulation, PLC-γ1 directly interacted with the cytoplasmic tail of integrin β1. In PLC-γ1-silenced cells, integrin-induced Pyk2 and paxillin phosphorylation were significantly reduced and PLC-γ1 potentiated the integrin-induced Pyk2/paxillin activation in its enzymatic activity-dependent manner. In addition, specific knock-down of PLC-γ1 resulted in a failure to form focal adhesions dependent on fibronectin stimulation, which appeared to be caused by the suppression of Pyk2 and paxillin phosphorylation. Interestingly, PLC-γ1 potentiated the activations of Rac, thus integrin-induced lamellipodia formation was up-regulated. Consequently, the strength of cell-substratum interaction and cell motility were profoundly up-regulated by PLC-γ1. Taken together, these results suggest that PLC-γ1 is a key player in integrin-mediated cell spreading and motility achieved by the activation of Pyk2/paxillin/Rac signaling.
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DOI
http://dx.doi.org/10.1016/j.cellsig.2007.04.002
ISSN
0898-6568
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