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Choi, Jang Hyun
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Phospholipase C-gamma 1 potentiates integrin-dependent cell spreading and migration through Pyk2/paxillin activation

Author(s)
Choi, Jang HyunYang, Yong-RyoulLee, Seul KiKim, Il-ShinHa, Sang HoonKim, Eung-KyunBae, Yun SooRyu, Sung HoSuh, Pann-Ghill
Issued Date
2007-08
DOI
10.1016/j.cellsig.2007.04.002
URI
https://scholarworks.unist.ac.kr/handle/201301/5661
Fulltext
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=34250639027
Citation
CELLULAR SIGNALLING, v.19, no.8, pp.1784 - 1796
Abstract
Phospholipase C-γ1 (PLC-γ1), which generates two second messengers, namely, inositol-1, 4, 5-trisphosphate and diacylglycerol, is implicated in growth factor-mediated chemotaxis. However, the exact role of PLC-γ1 in integrin-mediated cell adhesion and migration remains poorly understood. In this study, we demonstrate that PLC-γ1 is required for actin cytoskeletal organization and cell motility through the regulation of Pyk2 and paxillin activation. After fibronectin stimulation, PLC-γ1 directly interacted with the cytoplasmic tail of integrin β1. In PLC-γ1-silenced cells, integrin-induced Pyk2 and paxillin phosphorylation were significantly reduced and PLC-γ1 potentiated the integrin-induced Pyk2/paxillin activation in its enzymatic activity-dependent manner. In addition, specific knock-down of PLC-γ1 resulted in a failure to form focal adhesions dependent on fibronectin stimulation, which appeared to be caused by the suppression of Pyk2 and paxillin phosphorylation. Interestingly, PLC-γ1 potentiated the activations of Rac, thus integrin-induced lamellipodia formation was up-regulated. Consequently, the strength of cell-substratum interaction and cell motility were profoundly up-regulated by PLC-γ1. Taken together, these results suggest that PLC-γ1 is a key player in integrin-mediated cell spreading and motility achieved by the activation of Pyk2/paxillin/Rac signaling.
Publisher
ELSEVIER SCIENCE INC
ISSN
0898-6568
Keyword (Author)
phospholipase C-gamma lpyk2paxillinrac1integrinadhesionmigration
Keyword
FOCAL ADHESION KINASERICH TYROSINE KINASE-2GROWTH-FACTOR RECEPTORACTIN STRESS FIBERSC-GAMMA-LDIFFERENTIAL REGULATIONINVASIONPHOSPHORYLATIONMOTILITYRAC

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