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Suh, Pann-Ghill
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dc.citation.endPage 60 -
dc.citation.number 1 -
dc.citation.startPage 51 -
dc.citation.title PROTEOMICS -
dc.citation.volume 9 -
dc.contributor.author Yoon, Jong Hyuk -
dc.contributor.author Yea, Kyungmoo -
dc.contributor.author Kim, Jaeyoon -
dc.contributor.author Choi, Yoon Sup -
dc.contributor.author Park, Sehoon -
dc.contributor.author Lee, Hyeongji -
dc.contributor.author Lee, Chang Sup -
dc.contributor.author Suh, Pann-Ghill -
dc.contributor.author Ryu, Sung Ho -
dc.date.accessioned 2023-12-22T08:11:41Z -
dc.date.available 2023-12-22T08:11:41Z -
dc.date.created 2014-09-03 -
dc.date.issued 2009-01 -
dc.description.abstract Emerging evidence has revealed an endocrine function for skeletal muscle; in fact, certain antiinflammatory cytokines are secreted only from contractile skeletal muscle. However, the skeletal muscle secretome as a whole is poorly characterized, as is how it changes in response to extracellular stimuli. Herein, we sought to identify and characterize the members of the skeletal muscle secretome, and to determine which protein secretion levels were modulated in response to insulin stimulation. To conduct these studies, we treated differentiated L6 rat skeletal muscle cells with insulin or left them untreated, and we comparatively analyzed the proteins secreted into the media. We fractionated this conditioned media using offline RP HPLC, digested the fractionated proteins, and analyzed the resulting peptides with LC-ESI-MS/MS. We identified a total of 254 proteins, and by using three different filtering methods, we identified 153 of these as secretory proteins. Fourteen proteins were secreted at higher levels under insulin stimulation, including several proteins known to be highly secreted in metabolic diseases; 19 proteins were secreted at lower levels under insulin stimulation. These result not only pinpointed several previously unknown, insulin induced, secretory proteins of skeletal muscle, it also described a novel approach for conditioned secretome analysis. -
dc.identifier.bibliographicCitation PROTEOMICS, v.9, no.1, pp.51 - 60 -
dc.identifier.doi 10.1002/pmic.200800187 -
dc.identifier.issn 1615-9853 -
dc.identifier.scopusid 2-s2.0-59449096103 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/5652 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=59449096103 -
dc.identifier.wosid 000262568500006 -
dc.language 영어 -
dc.publisher WILEY-BLACKWELL -
dc.title Comparative proteomic analysis of the insulin-induced L6 myotube secretome -
dc.type Article -
dc.description.journalRegisteredClass scopus -
dc.subject.keywordAuthor Liquid chromatography-tandem mass spectrometry -
dc.subject.keywordAuthor Secreted protein -
dc.subject.keywordAuthor Shotgun proteomics -
dc.subject.keywordPlus PLASMINOGEN-ACTIVATOR INHIBITOR-1 -
dc.subject.keywordPlus SKELETAL-MUSCLE -
dc.subject.keywordPlus MATRIX METALLOPROTEINASES -
dc.subject.keywordPlus ADIPOSE-TISSUE -
dc.subject.keywordPlus DIABETES-MELLITUS -
dc.subject.keywordPlus IGF-I -
dc.subject.keywordPlus EXPRESSION -
dc.subject.keywordPlus GLUCOSE -
dc.subject.keywordPlus CELLS -
dc.subject.keywordPlus PROTEINS -

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