BROWSE

Related Researcher

Author

Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

ITEM VIEW & DOWNLOAD

Comparative analysis of the secretory proteome of human adipose stromal vascular fraction cells during adipogenesis

Cited 30 times inthomson ciCited 31 times inthomson ci
Title
Comparative analysis of the secretory proteome of human adipose stromal vascular fraction cells during adipogenesis
Author
Kim, JaeyoonChoi, Yoon SupLim, SeyoungYea, KyungmooYoon, Jong HyukJun, Dong-JaeHa, Sang HoonKim, Jung-WookKim, Jae HoSuh, Pann-GhillRyu, Sung HoLee, Taehoon G.
Keywords
Adipogenesis; Adipose stromal vascular fraction cells; Cell biology; Differentiation; LC/MS/MS; Secretome
Issue Date
201002
Publisher
WILEY-BLACKWELL
Citation
PROTEOMICS, v.10, no.3, pp.394 - 405
Abstract
Adipogenesis is a complex process that is accompanied by a number of molecular events. In this study, a proteomic approach was adopted to identify secretory factors associated with adipogenesis. A label-free shotgun proteomic strategy was implemented to analyze proteins secreted by human adipose stromal vascular fraction cells and differentiated adipocytes. A total of 474 proteins were finally identified and classified according to quantitative changes and statistical significances. Briefly, 177 proteins were significantly upregulated during adipogenesis (Class I), whereas 60 proteins were significantly downregulated (Class II). Changes in the expressions of several proteins were confirmed by quantitative RT-PCR and immunoblotting. One obvious finding based on proteomic data was that the amounts of several extracellular modulators of Wnt and transforming growth factor-β (TGF-β) signaling changed during adipogenesis. The expressions of secreted frizzled-related proteins, dickkopfrelated proteins, and latent TGF-β-binding proteins were found to be altered during adipogenesis, which suggests that they participate in the fine regulation of Wnt and TGF-β signaling. This study provides useful tools and important clues regarding the roles of secretory factors during adipogenic differentiation, and provides information related to obesity and obesity-related metabolic diseases.
URI
Go to Link
DOI
http://dx.doi.org/10.1002/pmic.200900218
ISSN
1615-9853
Appears in Collections:
SLS_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU