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Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Requirement for the L-type Ca2+ channel alpha(1D) subunit in postnatal pancreatic beta cell generation

Cited 79 times inthomson ciCited 81 times inthomson ci
Title
Requirement for the L-type Ca2+ channel alpha(1D) subunit in postnatal pancreatic beta cell generation
Author
Namkung, YSkrypnyk, NJeong, MJLee, TLee, MSKim, HLChin, HSuh, Pann-GhillKim, SSShin, HS
Keywords
INCREASED INSULIN SENSITIVITY; DEPENDENT CALCIUM-CHANNEL; ACTIVIN RECEPTOR; FUNCTIONAL EXPRESSION; DIABETES-MELLITUS; TRANSGENIC MICE; B-CELLS; SECRETION; GROWTH; ISLETS
Issue Date
200110
Publisher
AMER SOC CLINICAL INVESTIGATION INC
Citation
JOURNAL OF CLINICAL INVESTIGATION, v.108, no.7, pp.1015 - 1022
Abstract
Pancreatic 5 cells are the source of insulin, which directly lowers blood glucose levels in the body. Our analyses Of alpha (1D) gene-knockout (alpha (1D-/-)) mice show that the L-type calcium channel, alpha (1D), is required for proper beta cell generation in the postnatal pancreas. Knockout mice were characteristically slightly smaller than their littermates and exhibited hypoinsulinemia and glucose intolerance. However, isolated a(1D)(-/-) islets persisted in glucose sensing and insulin secretion, with compensatory overexpression of another L-type channel gene, alpha (1C). Histologically, newborn a(1D)(-/-) mice had an equivalent number of islets to wild-type mice. in contrast, adult alpha (-/-)(1D) mice showed a decrease in the number and size of islets, compared with littermate wild-type mice due to a decrease in beta cell generation. TUNEL staining showed that there was no increase in cell. death in alpha (-/-)(1D) islets, and a 5-bromo-2 ' deoxyuridine-labeling (BrdU-labeling) assay illustrated significant reduction in the proliferation rate of beta cells in alpha (-/-)(1D) islets.
URI
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DOI
http://dx.doi.org/10.1172/JCI13310
ISSN
0021-9738
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SLS_Journal Papers
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