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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | 566 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 564 | - |
dc.citation.title | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY | - |
dc.citation.volume | 58 | - |
dc.contributor.author | Jang, SB | - |
dc.contributor.author | Cho, YS | - |
dc.contributor.author | Eom, SJ | - |
dc.contributor.author | Choi, EJ | - |
dc.contributor.author | Kim, KH | - |
dc.contributor.author | Suh, Pann-Ghill | - |
dc.contributor.author | Oh, BH | - |
dc.date.accessioned | 2023-12-22T11:39:20Z | - |
dc.date.available | 2023-12-22T11:39:20Z | - |
dc.date.created | 2014-09-02 | - |
dc.date.issued | 2002-03 | - |
dc.description.abstract | SEDL (known also as sedlin) is a 140 amino-acid protein with a putative role in endoplasmic reticulum-to-Golgi transport. Several missense mutations and deletion mutations in the SEDL gene, which result in protein truncation by frame shift, are responsible for spondyloepiphyseal dysplasia tarda, a progressive skeletal disorder. The protein is identical to MIP-2A, which was shown to interact physically with c-myc promotor-binding protein 1 (MBP-1) and relieve the regulatory role of MBP-1 as a general transcription repressor. In order to gain insights into the function of SEDL by structural analysis, the protein was overexpressed and crystallized as a first step. SEDL was overexpressed in Escherichia coli and crystallized using the hanging-drop vapour-diffusion method at 298 K. The crystals belong to the orthorhombic space group C2221, with unit-cell parameters a = 46.69, b = 101.30, c = 66.15 A. The unit cell is likely to contain one molecule of SEDL, with a crystal volume per protein mass (VM) of 2.36 A3 Da-1 and a solvent content of about 47.9% by volume. A native data set to 2.8 A resolution was obtained from a flash-cooled crystal using synchrotron radiation. | - |
dc.identifier.bibliographicCitation | ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, v.58, no.3, pp.564 - 566 | - |
dc.identifier.doi | 10.1107/S0907444902001403 | - |
dc.identifier.issn | 0907-4449 | - |
dc.identifier.scopusid | 2-s2.0-0036514146 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/5615 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0036514146 | - |
dc.identifier.wosid | 000174227200036 | - |
dc.language | 영어 | - |
dc.publisher | WILEY-BLACKWELL | - |
dc.title | Crystallization and preliminary X-ray crystallographic analysis of SEDL | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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