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Author

Suh, Pann-Ghill
BioSignal Network Lab (BSN)
Research Interests
  • Signal transduction, cancer, metabolism, phospholipase C

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Determination of EGFR Endocytosis Kinetic by Auto-Regulatory Association of PLD1 with mu 2

Cited 3 times inthomson ciCited 5 times inthomson ci
Title
Determination of EGFR Endocytosis Kinetic by Auto-Regulatory Association of PLD1 with mu 2
Author
Lee, Jun SungKim, Il ShinKim, Jung HwanCho, WonhwaSuh, Pann-GhillRyu, Sung Ho
Issue Date
200909
Publisher
PUBLIC LIBRARY SCIENCE
Citation
PLOS ONE, v.4, no.9, pp.1 - 7
Abstract
Background: Upon ligand binding, cell surface signaling receptors are internalized through a process tightly regulated by endocytic proteins and adaptor protein 2 (AP2) to orchestrate them. Although the molecular identities and roles of endocytic proteins are becoming clearer, it is still unclear what determines the receptor endocytosis kinetics which is mainly regulated by the accumulation of endocytic apparatus to the activated receptors. Methodology/Principal Findings: Here we employed the kinetic analysis of endocytosis and adaptor recruitment to show that μ2, a subunit of AP2 interacts directly with phospholipase D (PLD)1, a receptor-associated signaling protein and this facilitates the membrane recruitment of AP2 and the endocytosis of epidermal growth factor receptor (EGFR). We also demonstrate that the PLD1-μ2 interaction requires the binding of PLD1 with phosphatidic acid, its own product. Conclusions/Significance: These results suggest that the temporal regulation of EGFR endocytosis is achieved by auto-regulatory PLD1 which senses the receptor activation and triggers the translocation of AP2 near to the activated receptor.
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DOI
http://dx.doi.org/10.1371/journal.pone.0007090
ISSN
1932-6203
Appears in Collections:
SLS_Journal Papers
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