Full metadata record
DC Field | Value | Language |
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dc.citation.number | 1 | - |
dc.citation.startPage | 67 | - |
dc.citation.title | MICROORGANISMS | - |
dc.citation.volume | 9 | - |
dc.contributor.author | Yang, Seung-Min | - |
dc.contributor.author | Baek, Jiwon | - |
dc.contributor.author | Kim, Eiseul | - |
dc.contributor.author | Kim, Hyeon-Be | - |
dc.contributor.author | Ko, Seyoung | - |
dc.contributor.author | Kim, Donghyuk | - |
dc.contributor.author | Yoon, Hyunjin | - |
dc.contributor.author | Kim, Hae-Yeong | - |
dc.date.accessioned | 2023-12-21T16:20:05Z | - |
dc.date.available | 2023-12-21T16:20:05Z | - |
dc.date.created | 2022-01-03 | - |
dc.date.issued | 2021-01 | - |
dc.description.abstract | In recent years, Salmonella Infantis has become a predominant serovariant in clinical and poultry isolates, thereby imposing a substantial economic burden on both public health and the livestock industry. With the aim of coping with the steep increase in serovar Infantis prevalence, a polymerase chain reaction (PCR)-based rapid and accurate diagnostic assay was developed in this study through pangenome profiling of 60 Salmonella serovars. A gene marker, SIN_02055, was identified, which is present in the S. Infantis genome but not in the pangenome of the other serovars. Primers specific to SIN_02055 were used to accurately detect serovar Infantis, and to successfully differentiate Infantis from the other 59 serovars in real-time PCR with a R-2 of 0.999 and an efficiency of 95.76%. The developed method was applied to 54 Salmonella strains belonging to eight dominant serovars, and distinguished Infantis from the other seven serovars with an accuracy of 100%. The diagnostic primer set also did not show false positive amplification with 32 strains from eight non-Salmonella bacterial species. This cost-effective and rapid method can be considered an alternative to the classic serotyping using antisera. | - |
dc.identifier.bibliographicCitation | MICROORGANISMS, v.9, no.1, pp.67 | - |
dc.identifier.doi | 10.3390/microorganisms9010067 | - |
dc.identifier.issn | 2076-2607 | - |
dc.identifier.scopusid | 2-s2.0-85098541575 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/55940 | - |
dc.identifier.url | https://www.mdpi.com/2076-2607/9/1/67 | - |
dc.identifier.wosid | 000610606200001 | - |
dc.language | 영어 | - |
dc.publisher | MDPI | - |
dc.title | Development of a Genoserotyping Method for Salmonella Infantis Detection on the Basis of Pangenome Analysis | - |
dc.type | Article | - |
dc.description.isOpenAccess | TRUE | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.type.docType | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
dc.subject.keywordAuthor | Salmonella Infantis | - |
dc.subject.keywordAuthor | genoserotyping | - |
dc.subject.keywordAuthor | pangenome | - |
dc.subject.keywordAuthor | real-time PCR | - |
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