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- Cho, Yoon-Kyoung
- FRUITS Lab.
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One step pathogen specific DNA extraction from whole blood on a centrifugal microfluidic device
- Author(s)
- Cho, Yoon-Kyoung, Cho, Y.-K., Lee, J.-G., Park, J.-M., Lee, B.-S., Lee, Y., Ko, C.
- Issued Date
- 2008-08-18
- Citation
- Systems Integration in Biodefense” conference
- Abstract
- We could, for the first time, demonstrate a fully integrated pathogen specific DNA extraction from whole blood utilizing centrifugal microfluidics on a polymer based CD platform. By combining the TS-LIMBS (Target Separation and Laser-Irradiated Magnetic Bead System) and centrifugal microfluidics using the novel LIFM (The Laser Irradiated Ferrowax Microvalves), DNA extraction experiments from whole blood spiked with Hepatitis B virus (HBV) were conducted. The total process was finished within 12 minutes with only one manual step of loading 100 μL of whole blood. Real-time PCR results showed that the concentration of DNA prepared on a CD was as good as that of the samples prepared in conventional bench top method.
- Publisher
- Cambridge Healthtech Institute
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