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권혁무

Kwon, Hyug Moo
Immunometabolism and Cancer Lab.
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dc.citation.endPage 662 -
dc.citation.number 2 -
dc.citation.startPage 656 -
dc.citation.title JOURNAL OF CLINICAL INVESTIGATION -
dc.citation.volume 88 -
dc.contributor.author UCHIDA, S -
dc.contributor.author NAKANISHI, T -
dc.contributor.author Kwon, H. Moo -
dc.contributor.author PRESTON, AS -
dc.contributor.author HANDLER, JS -
dc.date.accessioned 2023-12-22T13:08:12Z -
dc.date.available 2023-12-22T13:08:12Z -
dc.date.created 2014-06-03 -
dc.date.issued 1991-08 -
dc.description.abstract Using a clonal growth assay, we demonstrated that taurine, a nonperturbing osmolyte accumulated in kidney medulla, brain, and some other tissues of hypertonic experimental animals can function as a nonperturbing osmolyte in Madin-Darby canine kidney (MDCK) cells. The taurine content of hypertonic MDCK cells is twice that of isotonic MDCK cells (isotonic 160 nmol/mg protein; hypertonic 320 nmol/mg protein). Therefore we studied taurine transport in MDCK cells grown on porous supports and then studied the effect of hypertonicity which is known to elicit increased uptake of some other nonperturbing osmolytes by MDCK cells. Basal uptake exceeded apical uptake, with K(m) and V(max) of 56 μM and 933 pmol/min · mg protein on the basal surface and 10 μM and 50 pmol/min · mg protein on the apical surface. On both surfaces, virtually all taurine uptake was Na+ and Cl- dependent. 24 h after cells were shifted to hypertonic medium (500 mosmol/kg), taurine uptake doubled on the basolateral surface without change on the apical surface. The response to hypertonicity was the result of an increase in V(max) without change in K(m). There was no change in taurine efflux when cells were shifted from isotonic to hypertonic medium. When cells adapted to hypertonic medium were shifted to isotonic medium, a large transient basolateral efflux of taurine occurred within 10 min. We conclude that taurine can function as a nonperturbing osmolyte in MDCK cells and that tonicity-regulated taurine transport is a basolateral function in MDCK cells. -
dc.identifier.bibliographicCitation JOURNAL OF CLINICAL INVESTIGATION, v.88, no.2, pp.656 - 662 -
dc.identifier.doi 10.1172/JCI115350 -
dc.identifier.issn 0021-9738 -
dc.identifier.scopusid 2-s2.0-0025835489 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/4918 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025835489 -
dc.identifier.wosid A1991GA27600040 -
dc.language 영어 -
dc.publisher AMER SOC CLINICAL INVESTIGATION INC -
dc.title TAURINE BEHAVES AS AN OSMOLYTE IN MADIN-DARBY CANINE KIDNEY-CELLS - PROTECTION BY POLARIZED, REGULATED TRANSPORT OF TAURINE -
dc.type Article -
dc.description.journalRegisteredClass scopus -

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