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Author

Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

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TAURINE BEHAVES AS AN OSMOLYTE IN MADIN-DARBY CANINE KIDNEY-CELLS - PROTECTION BY POLARIZED, REGULATED TRANSPORT OF TAURINE

Cited 115 times inthomson ciCited 78 times inthomson ci
Title
TAURINE BEHAVES AS AN OSMOLYTE IN MADIN-DARBY CANINE KIDNEY-CELLS - PROTECTION BY POLARIZED, REGULATED TRANSPORT OF TAURINE
Author
UCHIDA, SNAKANISHI, TKwon, H. MooPRESTON, ASHANDLER, JS
Keywords
Chloride cotransport; Colony-forming efficiency; Cultured kidney cells; Sodium cotransport
Issue Date
199108
Publisher
AMER SOC CLINICAL INVESTIGATION INC
Citation
JOURNAL OF CLINICAL INVESTIGATION, v.88, no.2, pp.656 - 662
Abstract
Using a clonal growth assay, we demonstrated that taurine, a nonperturbing osmolyte accumulated in kidney medulla, brain, and some other tissues of hypertonic experimental animals can function as a nonperturbing osmolyte in Madin-Darby canine kidney (MDCK) cells. The taurine content of hypertonic MDCK cells is twice that of isotonic MDCK cells (isotonic 160 nmol/mg protein; hypertonic 320 nmol/mg protein). Therefore we studied taurine transport in MDCK cells grown on porous supports and then studied the effect of hypertonicity which is known to elicit increased uptake of some other nonperturbing osmolytes by MDCK cells. Basal uptake exceeded apical uptake, with K(m) and V(max) of 56 μM and 933 pmol/min · mg protein on the basal surface and 10 μM and 50 pmol/min · mg protein on the apical surface. On both surfaces, virtually all taurine uptake was Na+ and Cl- dependent. 24 h after cells were shifted to hypertonic medium (500 mosmol/kg), taurine uptake doubled on the basolateral surface without change on the apical surface. The response to hypertonicity was the result of an increase in V(max) without change in K(m). There was no change in taurine efflux when cells were shifted from isotonic to hypertonic medium. When cells adapted to hypertonic medium were shifted to isotonic medium, a large transient basolateral efflux of taurine occurred within 10 min. We conclude that taurine can function as a nonperturbing osmolyte in MDCK cells and that tonicity-regulated taurine transport is a basolateral function in MDCK cells.
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DOI
http://dx.doi.org/10.1172/JCI115350
ISSN
0021-9738
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SLS_Journal Papers
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