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Author

Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

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THE TONICITY-SENSITIVE ELEMENT THAT MEDIATES INCREASED TRANSCRIPTION OF THE BETAINE TRANSPORTER GENE IN RESPONSE TO HYPERTONIC STRESS

Cited 168 times inthomson ciCited 159 times inthomson ci
Title
THE TONICITY-SENSITIVE ELEMENT THAT MEDIATES INCREASED TRANSCRIPTION OF THE BETAINE TRANSPORTER GENE IN RESPONSE TO HYPERTONIC STRESS
Author
TAKENAKA, MPRESTON, ASKwon, H. MooHANDLER, JS
Keywords
INOSITOL COTRANSPORTER; OSMOTIC REGULATION; ESCHERICHIA-COLI; ALDOSE REDUCTASE; CELL LINES; MDCK CELLS; PROTEIN; CLONING; MYOINOSITOL; PHYSIOLOGY
Issue Date
199411
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, v.269, no.47, pp.29379 - 29381
Abstract
BGT1, the Na+- and Cl--coupled betaine transporter, is responsible for the accumulation of high concentrations of the non-perturbing osmolyte betaine in hypertonic Madin-Darby canine kidney (MDCK) cells and presumably in the hypertonic renal medulla. In MDCK cells, the increase in activity of the betaine transporter is preceded by an increase in transcription of BGT1 and in the abundance of BGT1 mRNA. To investigate the molecular mechanism of transcriptional regulation by tonicity, we have characterized the 5'-flanking region of the gene. Transient transfection assays in MDCK cells cultured in isotonic or hypertonic medium using luciferase reporter constructs containing various fragments of the 5'-flanking region revealed that the region spanning base pairs -69 to -50 5' to the transcription initiation site (-69/-50) has hypertonicity-responsive enhancer activity. A double-stranded -69/-50 concatemer cloned 5' to an SV40 basal promoter and luciferase reporter gene in hypertonic cells exhibited more than 11-fold the activity in isotonic cells. Expression assays and electrophoretic mobility shift assays of mutants of -69/-50 identified a smaller region that is required for hypertonicity to induce increased expression and a slowly migrating band on mobility shift assays.
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ISSN
0021-9258
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