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DC Field | Value | Language |
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dc.citation.endPage | 5760 | - |
dc.citation.number | 16 | - |
dc.citation.startPage | 5753 | - |
dc.citation.title | MOLECULAR AND CELLULAR BIOLOGY | - |
dc.citation.volume | 22 | - |
dc.contributor.author | Woo, SK | - |
dc.contributor.author | Lee, SD | - |
dc.contributor.author | Na, KY | - |
dc.contributor.author | Park, WK | - |
dc.contributor.author | Kwon, H. Moo | - |
dc.date.accessioned | 2023-12-22T11:37:22Z | - |
dc.date.available | 2023-12-22T11:37:22Z | - |
dc.date.created | 2014-06-05 | - |
dc.date.issued | 2002-08 | - |
dc.description.abstract | While hyperosmolality of the kidney medulla is essential for urinary concentration, it imposes a great deal of stress. Cells in the renal medulla adapt to the stress of hypertonicity (hyperosmotic salt) by accumulating organic osmolytes. Tonicity-responsive enhancer (TonE) binding protein (TonEBP) (or NFAT5) stimulates transcription of transporters and a synthetic enzyme for the cellular accumulation of organic osmolytes. We found that dominant-negative TonEBP reduced expression of HSP70 as well as the transporters and enzyme. Near the major histocompatibility complex class III locus, there are three HSP70 genes named HSP70-1, HSP70-2, and HSC70t. While HSP70-1 and HSP70-2 were heat inducible, only HSP70-2 was induced by hypertonicity. In the 5' flanking region of the HSP70-2 gene, there are three sites for TonEBP binding. In cells transfected with a reporter plasmid containing this region, expression of luciferase was markedly stimulated in response to hypertonicity. Coexpression of the dominant-negative TonEBP reduced the luciferase expression. Mutating all three sites in the reporter plasmid led to a complete loss of induction by hypertonicity. Thus, TonEBP rather than heat shock factor stimulates transcription of the HSP70-2 gene in response to hypertonicity. We conclude that TonEBP is a master regulator of the renal medulla for cellular protection against high osmolality via organic osmolytes and molecular chaperones. | - |
dc.identifier.bibliographicCitation | MOLECULAR AND CELLULAR BIOLOGY, v.22, no.16, pp.5753 - 5760 | - |
dc.identifier.doi | 10.1128/MCB.16.22.5753-5760.2002 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.scopusid | 2-s2.0-0036318568 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/4904 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0036318568 | - |
dc.identifier.wosid | 000177089200011 | - |
dc.language | 영어 | - |
dc.publisher | AMER SOC MICROBIOLOGY | - |
dc.title | TonEBP/NFAT5 stimulates transcription of HSP70 in response to hypertonicity | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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