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권혁무

Kwon, Hyug Moo
Immunometabolism and Cancer Lab.
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dc.citation.endPage 5760 -
dc.citation.number 16 -
dc.citation.startPage 5753 -
dc.citation.title MOLECULAR AND CELLULAR BIOLOGY -
dc.citation.volume 22 -
dc.contributor.author Woo, SK -
dc.contributor.author Lee, SD -
dc.contributor.author Na, KY -
dc.contributor.author Park, WK -
dc.contributor.author Kwon, H. Moo -
dc.date.accessioned 2023-12-22T11:37:22Z -
dc.date.available 2023-12-22T11:37:22Z -
dc.date.created 2014-06-05 -
dc.date.issued 2002-08 -
dc.description.abstract While hyperosmolality of the kidney medulla is essential for urinary concentration, it imposes a great deal of stress. Cells in the renal medulla adapt to the stress of hypertonicity (hyperosmotic salt) by accumulating organic osmolytes. Tonicity-responsive enhancer (TonE) binding protein (TonEBP) (or NFAT5) stimulates transcription of transporters and a synthetic enzyme for the cellular accumulation of organic osmolytes. We found that dominant-negative TonEBP reduced expression of HSP70 as well as the transporters and enzyme. Near the major histocompatibility complex class III locus, there are three HSP70 genes named HSP70-1, HSP70-2, and HSC70t. While HSP70-1 and HSP70-2 were heat inducible, only HSP70-2 was induced by hypertonicity. In the 5' flanking region of the HSP70-2 gene, there are three sites for TonEBP binding. In cells transfected with a reporter plasmid containing this region, expression of luciferase was markedly stimulated in response to hypertonicity. Coexpression of the dominant-negative TonEBP reduced the luciferase expression. Mutating all three sites in the reporter plasmid led to a complete loss of induction by hypertonicity. Thus, TonEBP rather than heat shock factor stimulates transcription of the HSP70-2 gene in response to hypertonicity. We conclude that TonEBP is a master regulator of the renal medulla for cellular protection against high osmolality via organic osmolytes and molecular chaperones. -
dc.identifier.bibliographicCitation MOLECULAR AND CELLULAR BIOLOGY, v.22, no.16, pp.5753 - 5760 -
dc.identifier.doi 10.1128/MCB.16.22.5753-5760.2002 -
dc.identifier.issn 0270-7306 -
dc.identifier.scopusid 2-s2.0-0036318568 -
dc.identifier.uri https://scholarworks.unist.ac.kr/handle/201301/4904 -
dc.identifier.url http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0036318568 -
dc.identifier.wosid 000177089200011 -
dc.language 영어 -
dc.publisher AMER SOC MICROBIOLOGY -
dc.title TonEBP/NFAT5 stimulates transcription of HSP70 in response to hypertonicity -
dc.type Article -
dc.description.journalRegisteredClass scopus -

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