BROWSE

Related Researcher

Author

Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

ITEM VIEW & DOWNLOAD

Regulation of TonEBP transcriptional activator in MDCK cells following changes in ambient tonicity

Cited 34 times inthomson ciCited 34 times inthomson ci
Title
Regulation of TonEBP transcriptional activator in MDCK cells following changes in ambient tonicity
Author
Neuhofer, WWoo, SKNa, KYGrunbein, RPark, WKNahm, OBeck, FXKwon, H. Moo
Keywords
cell ionic strength; cell volume; organic osmolytes; heat shock protein 70; Madin-Darby canine kidney cells
Issue Date
200212
Publisher
AMER PHYSIOLOGICAL SOC
Citation
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, v.283, no.6, pp.C1604 - C1611
Abstract
In response to ambient hypertonicity, TonEBP (tonicity-responsive enhancer binding protein) stimulates certain genes including those encoding cytokines, transporters for organic solutes, and a molecular chaperone. TonEBP is regulated in a bidirectional manner, upregulated by an increase in ambient tonicity while downregulated by a decrease. To investigate the role of intracellular ionic strength in the activity of TonEBP, we subjected Madin-Darby canine kidney cells to a variety of conditions. Electron microprobe analysis was performed to measure intracellular electrolytes. Under conditions in which changes in cell volume were similar, TonEBP activity correlated with the intracellular ionic strength regardless of the external tonicity. On the other hand, inhibition of the Na+/K+-ATPase and high external K+ concentration led to a decreased activity of TonEBP despite a marked increase in the intracellular ionic strength. Because isotonic swelling is known to occur under these conditions, these data suggest that dilution of the cytoplasmic constituents inhibits the activity of TonEBP. We conclude that intracellular ionic strength and water content are major factors that determine the activity of TonEBP.
URI
Go to Link
DOI
http://dx.doi.org/10.1152/ajpcell.00216.2002
ISSN
0363-6143
Appears in Collections:
SLS_Journal Papers

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU