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Kwon, Hyug Moo
Immunometabolism and Cancer Lab.
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TonEBP is inhibited by RNA helicase A via interaction involving the E ' F loop

Author(s)
Colla, ELee, SDSheen, MRWoo, SKKwon, H. Moo
Issued Date
2006-01
DOI
10.1042/BJ20051082
URI
https://scholarworks.unist.ac.kr/handle/201301/4894
Fulltext
http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=30044437019
Citation
BIOCHEMICAL JOURNAL, v.393, pp.411 - 419
Abstract
TonEBP [TonE (tonicity-responsive enhancer)-binding protein] is a transcriptional activator of the Rel family like NF-κ B (nuclear factor κ B) and NFAT (nuclear factor of activated T-cells). TonEBP plays a key role in the protection of cells in the kidney medulla from the deleterious effects of hyperosmolality. This is achieved by enhancing expression of HSP70 (heat-shock protein 70) and other genes whose products drive cellular accumulation of organic osmolytes. TonEBP is stimulated by ambient hypertonicity via multiple pathways that regulate nuclear translocation and transactivation. In the present paper, we report that TonEBP is associated in vivo with RHA (RNA helicase A). The N- and C-termini of RHA bound the E′F loop of the DNA-binding domain of TonEBP. The interaction was not affected by DNA binding or dimerization of TonEBP. Overexpression of RHA inhibited the activity of TonEBP; however, catalytic activity of RHA was dispensable for the inhibition. When the ambient tonicity was raised, the TonEBP-RHA interaction decreased, suggesting that dissociation of RHA is a pathway to stimulate TonEBP. We conclude that the E′F loop of TonEBP interacts with RHA like NFAT and NF-κ B interact with AP1 (activator protein 1) and the high-mobility group protein HMG-I(Y) respectively. While RHA interacts with and stimulates other transcription factors such as CREB (cAMP-response-element-binding protein), NF-κ B and mineralocorticoid receptor, it inhibits TonEBP.
Publisher
PORTLAND PRESS LTD
ISSN
0264-6021
Keyword (Author)
E &aposF loophypertonicitynuclear factor kappa B (NF-kappa B)RNA helicase A (RHA)small interfering RNA (siRNA)tonicity-responsive-enhancer-binding protein (TonEBP)
Keyword
ENHANCER-BINDING PROTEINTRANSCRIPTION FACTORGENE-EXPRESSIONPOLYMERASE-IIDNA-BINDINGKAPPA-BTONICITYCOMPLEXHYPERTONICITYNFAT5

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