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DC Field | Value | Language |
---|---|---|
dc.citation.endPage | F202 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | F197 | - |
dc.citation.title | AMERICAN JOURNAL OF PHYSIOLOGY | - |
dc.citation.volume | 261 | - |
dc.contributor.author | YAMAUCHI, A | - |
dc.contributor.author | Kwon, H. Moo | - |
dc.contributor.author | UCHIDA, S | - |
dc.contributor.author | PRESTON, AS | - |
dc.contributor.author | HANDLER, JS | - |
dc.date.accessioned | 2023-12-22T13:08:15Z | - |
dc.date.available | 2023-12-22T13:08:15Z | - |
dc.date.created | 2014-06-02 | - |
dc.date.issued | 1991-07 | - |
dc.description.abstract | Myo-inositol and glycinebetaine are compatible osmolytes accumulated in the renal medulla and in MDCK cells cultured in hypertonic media. Both osmolytes are taken up by MDCK cells on Na-coupled transporters. The maximal velocity (V(max)) of both cotransporters is increased by culture in hypertonic medium. When hypertonic MDCK cells are shifted to isotonic medium there is a large transient efflux of osmolytes. To determine the polarity of the cotransporters and the transient efflux, we grew MDCK cells on a porous support to assay transport separately at their apical and basolateral surfaces. In hypertonic cells, basolateral uptake of both osmolytes was 1) more than 10-fold apical uptake, 2) > 96% Na dependent, 3) 25- (myo-inositol) and 16-fold (glycinebetaine) uptake in isotonic cells, reaching a maximum 24 h after the switch to hypertonic medium. When medium osmolarity was decreased from hypertonic to isotonic, myo-inositol uptake reversed to the isotonic level within 1 day; glycinebetaine uptake decreased more slowly. When medium osmolarity was decreased from hypertonic to isotonic, there was a large transient increase in basolateral efflux of both osmolytes. | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY, v.261, no.1, pp.F197 - F202 | - |
dc.identifier.issn | 0002-9513 | - |
dc.identifier.scopusid | 2-s2.0-0025874198 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/4865 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025874198 | - |
dc.identifier.wosid | A1991FY35700096 | - |
dc.language | 영어 | - |
dc.publisher | American Physiological Society | - |
dc.title | MYOINOSITOL AND BETAINE TRANSPORTERS REGULATED BY TONICITY ARE BASOLATERAL IN MDCK CELLS | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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