MYOINOSITOL AND BETAINE TRANSPORTERS REGULATED BY TONICITY ARE BASOLATERAL IN MDCK CELLS
Cited 112 times inCited 0 times in
- MYOINOSITOL AND BETAINE TRANSPORTERS REGULATED BY TONICITY ARE BASOLATERAL IN MDCK CELLS
- YAMAUCHI, A; Kwon, H. Moo; UCHIDA, S; PRESTON, AS; HANDLER, JS
- OSMOLYTES; CULTURED KIDNEY CELLS; SODIUM-DEPENDENT TRANSPORT; COTRANSPORTERS; PHLORIZIN; 4-ACETOMIDO-4'-ISOTHIOCYANOSTILBENE-2,2'-DISULFONIC ACID; 4,4'-DIISOTHIOCYANOSTIBENE-2,2'-DISULFONIC ACID; POLARITY
- Issue Date
- American Physiological Society
- AMERICAN JOURNAL OF PHYSIOLOGY, v.261, no.1, pp.F197 - F202
- Myo-inositol and glycinebetaine are compatible osmolytes accumulated in the renal medulla and in MDCK cells cultured in hypertonic media. Both osmolytes are taken up by MDCK cells on Na-coupled transporters. The maximal velocity (V(max)) of both cotransporters is increased by culture in hypertonic medium. When hypertonic MDCK cells are shifted to isotonic medium there is a large transient efflux of osmolytes. To determine the polarity of the cotransporters and the transient efflux, we grew MDCK cells on a porous support to assay transport separately at their apical and basolateral surfaces. In hypertonic cells, basolateral uptake of both osmolytes was 1) more than 10-fold apical uptake, 2) > 96% Na dependent, 3) 25- (myo-inositol) and 16-fold (glycinebetaine) uptake in isotonic cells, reaching a maximum 24 h after the switch to hypertonic medium. When medium osmolarity was decreased from hypertonic to isotonic, myo-inositol uptake reversed to the isotonic level within 1 day; glycinebetaine uptake decreased more slowly. When medium osmolarity was decreased from hypertonic to isotonic, there was a large transient increase in basolateral efflux of both osmolytes.
Appears in Collections:
- SLS_Journal Papers
- Files in This Item:
- There are no files associated with this item.
can give you direct access to the published full text of this article. (UNISTARs only)
Show full item record
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.