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Author

Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

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MEDIUM TONICITY REGULATES EXPRESSION OF THE NA+ DEPENDENT AND CL--DEPENDENT BETAINE TRANSPORTER IN MADIN-DARBY CANINE KIDNEY-CELLS BY INCREASING TRANSCRIPTION OF THE TRANSPORTER GENE

Cited 119 times inthomson ciCited 100 times inthomson ci
Title
MEDIUM TONICITY REGULATES EXPRESSION OF THE NA+ DEPENDENT AND CL--DEPENDENT BETAINE TRANSPORTER IN MADIN-DARBY CANINE KIDNEY-CELLS BY INCREASING TRANSCRIPTION OF THE TRANSPORTER GENE
Author
UCHIDA, SYAMAUCHI, APRESTON, ASKwon, H. MooHANDLER, JS
Keywords
Cell volume regulation; Cotransport; Hypertonicity; Organic osmolytes
Issue Date
199304
Publisher
AMER SOC CLINICAL INVESTIGATION INC
Citation
JOURNAL OF CLINICAL INVESTIGATION, v.91, no.4, pp.1604 - 1607
Abstract
Betaine is one of the major compatible osmolytes accumulated by kidney derived Madin-Darby canine kidney cells cultured in hypertonic medium. Betaine is accumulated by Na+- and Cl--dependent uptake from the medium. To gain insight into the mechanism by which hypertonicity evokes an increase in the V max of the betaine transporter in Madin-Darby canine kidney cells, we measured the relative abundance of mRNA for the transporter in cells shifted to a hypertonic medium and found parallel increases in mRNA abundance and cotransporter activity. The increase in mRNA levels preceded the increase in trans-porter activity slightly. Transcription of the gene for the transporter rose rapidly and to the same relative extent as mRNA abundance in cells shifted to hypertonic medium, indicating that transcription of the gene for the cotransporter plays a major role in regulating the accumulation of betaine in response to hypertonicity.
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DOI
http://dx.doi.org/10.1172/JCI116367
ISSN
0021-9738
Appears in Collections:
SLS_Journal Papers
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