BROWSE

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Author

Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

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Osmolarity in renal medulla of transgenic mice regulates transcription via 5'-flanking region of canine BGT1 gene

Cited 27 times inthomson ciCited 27 times inthomson ci
Title
Osmolarity in renal medulla of transgenic mice regulates transcription via 5'-flanking region of canine BGT1 gene
Author
Kaneko, TTakenaka, MOkabe, MYoshimura, YYamauchi, AHorio, MKwon, H. MooHandler, JSImai, E
Keywords
Kidney;  Osmolyte;  Tonicity-responsive enhancer
Issue Date
199705
Publisher
AMER PHYSIOLOGICAL SOC
Citation
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, v.272, no.5, pp.F610 - F616
Abstract
Betaine is a major compatible osmolyte accumulated in the mammalian kidney medulla and in Madin-Darby canine kidney cells in response to hypertonicity. The accumulation is the result of an increase in maximal velocity of the Na+- and Cl-coupled betaine transporter designated BGT1. We have previously cloned the canine BGT1 gene and identified a tonicity- responsive enhancer element (TonE) in its 5'-flanking region. Here we report studies of transgenic mice that have in their genome 2.4 kb of the 5'- flanking region of the canine BGT1 gene in front of a chloramphenicol acetyl- transferase (CAT) reporter. Expression of CAT mRNA was detected only in the renal medulla and was increased by experimental manipulations that increase the tonicity of the renal medulla and decreased by manipulations that decrease medullary tonicity. We conclude that the 2.4-kb 5'-flanking region of the BGT1 gene mediates an increase in transcription in response to hyperosmolarity in the renal medulla.
URI
http://scholarworks.unist.ac.kr/handle/201301/4858
ISSN
1931-857X
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SLS_Journal Papers
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