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Author

Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

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Nuclear redistribution of tonicity-responsive enhancer binding protein requires proteasome activity

Cited 42 times inthomson ciCited 43 times inthomson ci
Title
Nuclear redistribution of tonicity-responsive enhancer binding protein requires proteasome activity
Author
Woo, SKMaouyo, DHandler, JSKwon, H. Moo
Keywords
Hypertonicity-stimulated transcription; Sodium-chloride-betaine cotransporter; Sodium-myo-inositol cotransporter
Issue Date
200002
Publisher
AMER PHYSIOLOGICAL SOC
Citation
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY, v.278, no.2, pp.C323 - C330
Abstract
Tonicity-responsive enhancer binding protein (TonEBP) is the transcription factor that regulates tonicity-responsive expression of the genes for the sodium-myo-inositol cotransporter (SMIT) and the sodium- chloride-betaine cotransporter (BGT1). Hypertonicity stimulates the activity of TonEBP due to a combination of increased protein abundance and increased nuclear distribution (proportion of TonEBP that is in the nucleus). We found that inhibitors of proteasome activity markedly reduce the induction of SMIT and BGT1 mRNA in response to hypertonicity. These inhibitors also reduce hypertonicity-induced stimulation of expression of a reporter gene controlled by the tonicity-responsive enhancer. Western and immunohistochemical analyses revealed that the proteasome inhibitors reduce the hypertonicity-induced increase of TonEBP in the nucleus by inhibiting its nuclear redistribution without affecting its abundance. Although the nuclear distribution of TonEBP is sensitive to inhibition of proteasome activity as is that of nuclear factor (NF)-κB, the signaling pathways appear to be different in that hypertonicity does not affect the nuclear distribution of NF-κB. Conversely, treatment with tumor necrosis factor-α increases the nuclear distribution of NF-κB but not TonEBP.
URI
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ISSN
0363-6143
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SLS_Journal Papers

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