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Author

Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

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Expression of rat fibroblast growth factor receptor 1 as three splicing variants during kidney development

Cited 17 times inthomson ciCited 16 times inthomson ci
Title
Expression of rat fibroblast growth factor receptor 1 as three splicing variants during kidney development
Author
Kim, Eung GookKwon, H. MooBurrow, Christopher R.Ballermann, Barbara J.
Keywords
angiogenesis;  embryogenesis;  polymerase chain reaction;  ribonucleic acid splicing
Issue Date
199301
Publisher
American Physiological Society
Citation
AMERICAN JOURNAL OF PHYSIOLOGY, v.264, no.1, pp.F66 - F73
Abstract
Fibroblast growth factors (FGF) are known to participate in the processes of embryogenesis and angiogenesis. This study was undertaken to examine the transcriptional and posttranscriptional regulation of the FGF receptor 1 (FGFR-1) subclass in the embryonic rat kidney. Two full-length FGF receptor cDNAs were cloned using low-stringency screening of a neonatal rat kidney library with a chicken FGFR-1 cDNA probe. Sequencing revealed these cloned cDNAs to be rat homologues of the FGFR-1 subtype, with the two clones representing splicing variants β and γ of the FGFR-1. Evidence for renal expression of a third splicing variant (α) was obtained by use of the polymerase chain reaction. Splicing variants α and β of FGFR-1 are predicted to produce cell-surface FGF receptors with three and two immunoglobulin-like domains, respectively, whereas the γ-isoform may represent an intracellular form of the receptor. Although all three splicing variants were expressed in the developing kidney at days 14, 17, and 20 of gestation, at neonatal days 1 and 7 and in mature rats the β-isoform was present in vastly larger abundance than α- and γ-isoforms at all stages studied. Northern blot analysis revealed enhanced expression of FGFR-1 in the neonatal compared with the mature kidney. It is concluded that FGFR-1 is expressed in the kidney predominantly as the β-isoform splicing variant and that expression of this receptor is enhanced during kidney development.
URI
http://scholarworks.unist.ac.kr/handle/201301/4832
ISSN
0002-9513
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