BROWSE

Related Researcher

Author

Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

ITEM VIEW & DOWNLOAD

HYPERTONICITY STIMULATES TRANSCRIPTION OF GENE FOR NA+-MYO-INOSITOL COTRANSPORTER IN MDCK CELLS

Cited 124 times inthomson ciCited 101 times inthomson ci
Title
HYPERTONICITY STIMULATES TRANSCRIPTION OF GENE FOR NA+-MYO-INOSITOL COTRANSPORTER IN MDCK CELLS
Author
YAMAUCHI, AUCHIDA, SPRESTON, ASKwon, H. MooHANDLER, JS
Keywords
Messenger ribonucleic acid abundance;  Osmolytes;  Sodium-dependent transport
Issue Date
199301
Publisher
American Physiological Society
Citation
AMERICAN JOURNAL OF PHYSIOLOGY, v.264, no.1, pp.F20 - F23
Abstract
Myo-inositol is a major compatible osmolyte accumulated in the hypertonic renal medulla and in Madin-Darby canine kidney (MDCK) cells cultured in hypertonic media. Myo-inositol is taken up by MDCK cells on a Na+-coupled transporter whose activity increases six-fold 24 h after cells are switched to hypertonic medium. To investigate the mechanism of regulation of the cotransporter by hypertonicity, we used the cDNA encoding the canine Na+-myo-inositol cotransporter that we recently cloned to measure the abundance of the mRNA for the cotransporter and its rate of transcription after changes in osmolality. When MDCK cells were switched from isotonic to hypertonic medium, cotransporter mRNA abundance rose 10-fold in 16 h. Transcription of the cotransporter gene also rose and 16 h after the switch reached a peak ∼15-fold that in isotonic cells. When cells were switched back to isotonic medium, mRNA abundance and transcription of the gene returned to isotonic levels in 8 h and transport rate reached isotonic levels in 48 h. Thus transcription appears to be the primary step in regulation of myo-inositol transport by hypertonicity.
URI
http://scholarworks.unist.ac.kr/handle/201301/4831
ISSN
0002-9513
Appears in Collections:
SLS_Journal Papers
Files in This Item:
There are no files associated with this item.

find_unist can give you direct access to the published full text of this article. (UNISTARs only)

Show full item record

qr_code

  • mendeley

    citeulike

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

MENU