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Author

Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

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Induction of glycinebetaine uptake into xenopus oocytes by injection of poly(A)+ RNA from renal cells exposed to high extracellular NaCl

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Title
Induction of glycinebetaine uptake into xenopus oocytes by injection of poly(A)+ RNA from renal cells exposed to high extracellular NaCl
Author
ROBEY, RBKwon, H. MooHANDLER, JSGARCIAPEREZ, ABURG, MB
Keywords
ACTIVE RIBONUCLEIC-ACID; MAJOR ORGANIC OSMOLYTES; MESSENGER-RNA; ALDOSE REDUCTASE; MEDULLARY CELLS; LAEVIS OOCYTES; MOLECULAR-CLONING; OSMOTIC-STRESS; EXPRESSION; RAT
Issue Date
199106
Publisher
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Citation
JOURNAL OF BIOLOGICAL CHEMISTRY, v.266, no.16, pp.10400 - 10405
Abstract
Madin-Darby canine kidney (MDCK) cells accumulate glycinebetaine via Na+-dependent transport in response to hypertonic stress. When extracellular tonicity is increased by the addition of NaCl, V(max) for glycinebetaine transport increases without an associated change in K(m), consistent with an increase in the number of functioning transporters. To test whether increased transport activity results from increased gene expression, we injected poly(A)+ RNA (mRNA) from MDCK cells into Xenopus oocytes and assayed for glycinebetaine uptake in ovo. RNA-induced Na+-dependent uptake is observed in oocytes injected with mRNA from cells exposed to high extracellular NaCl, but not in oocytes injected with either water or mRNA from cells maintained in isotonic medium. Unfractionated mRNA induces glycinebetaine uptake in ovo at a rate which is approximately 3-fold higher than in water-injected controls. Size-fractionated mRNA (median size 2.8 kilobases) induces uptake at a rate which is approximately 7-fold higher than controls. Such RNA-induced transport activity in ovo is consistent with heterologous expression of Na+/glycinebetaine cotransporters encoded by renal mRNA. Increased transporter mRNA in cells exposed to hypertonicity probably underlies the pattern of expression observed in ovo. This can account for the observed rise in MDCK cell glycinebetaine transport during hypertonic stress.
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ISSN
0021-9258
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