BROWSE

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Author

Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

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Hydration status affects nuclear distribution of transcription factor tonicity responsive enhancer binding protein in rat kidney

Cited 51 times inthomson ciCited 53 times inthomson ci
Title
Hydration status affects nuclear distribution of transcription factor tonicity responsive enhancer binding protein in rat kidney
Author
Cha, JHWoo, SKHan, KHKim, YHHandler, JSKim, JKwon, H. Moo
Keywords
COTRANSPORTER MESSENGER-RNA; ALDOSE REDUCTASE; INOSITOL COTRANSPORTER; OSMOTIC-STRESS; MEDULLA; GENE; IDENTIFICATION; HYPERTONICITY; OSMOLYTES; CLONING
Issue Date
200111
Publisher
AMER SOC NEPHROLOGY
Citation
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, v.12, no.11, pp.2221 - 2230
Abstract
Tonicity responsive enhancer binding protein (TonEBP) is the transcription factor that regulates tonicity responsive expression of proteins that catalyze cellular accumulation of compatible osmolytes. In cultured MDCK cells, hypertonicity stimulates the activity of TonEBP via a combination of increased protein abundance and increased nuclear localization. For investigating regulation of TonEBP in the kidney, rats were subjected to water loading or dehydration. Water loading lowered urine osmolality and mRNA expression of sodium/myo-inositol cotransporter (SMIT), a target gene of TonEBP, in the renal medulla; dehydration doubled the urine osmolality and increased SMIT mRNA expression. In contrast, overall abundance of TonEBP and its mRNA measured by immunoblot and ribonuclease protection assay, respectively, was not affected. Immunohistochemical analysis, however, revealed that nuclear distribution of TonEBP is generally increased throughout the medulla in dehydrated animals compared with water loaded animals. Increased nuclear localization was particularly dramatic in thin limbs. Notable exceptions were the middle to terminal portions of the inner medullary collecting ducts and blood vessels, where a change in TonEBP distribution was not evident. Immunohistochemical detection of SMIT mRNA revealed that the changes in nuclear distribution of TonEBP correlate with expression of SMIT. It is concluded that under physiologic conditions, nucleocytoplasmic distribution is the dominant mode of regulation of TonEBP in the renal medulla.
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ISSN
1046-6673
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