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DC Field | Value | Language |
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dc.citation.endPage | 9609 | - |
dc.citation.number | 15 | - |
dc.citation.startPage | 9605 | - |
dc.citation.title | JOURNAL OF BIOLOGICAL CHEMISTRY | - |
dc.citation.volume | 266 | - |
dc.contributor.author | UCHIDA, S | - |
dc.contributor.author | Kwon, H. Moo | - |
dc.contributor.author | PRESTON, AS | - |
dc.contributor.author | HANDLER, JS | - |
dc.date.accessioned | 2023-12-22T13:08:23Z | - |
dc.date.available | 2023-12-22T13:08:23Z | - |
dc.date.created | 2014-05-23 | - |
dc.date.issued | 1991-05 | - |
dc.description.abstract | Expression of a Madin-Darby canine kidney (MDCK) cell taurine transporter was examined in Xenopus oocytes that had been injected with poly(A)+ RNA extracted from MDCK cells. Compared with water-injected oocytes, injection of total poly(A)+ RNA resulted in an increase in Na+-dependent taurine uptake which was directly related to the amount of RNA injected. The magnitude of expression in poly(A)+ RNA-injected oocytes was 5-10-fold higher than that of water-injected oocytes. Since the Vmax of taurine uptake in MDCK cells is increased by culture in hypertonic medium, we compared oocyte taurine uptake after injection with poly(A)+ RNA from MDCK cells cultured in hypertonic medium with uptake in oocytes injected with poly(A)+ RNA from MDCK cells cultured in isotonic medium. Poly(A)+ RNA from hypertonic cells elicited twice the taurine uptake elicited by poly(A)+ RNA from isotonic cells. The transporter expressed in oocytes was like that in MDCK cells: it was completely dependent on external sodium and was also anion dependent (Cl- ≥ Br- > SCN- ≫ gluconate-). Other β-amino acids, β-alanine and hypotaurine, inhibited taurine uptake, but L-alanine and 2-(methylamino) isobutyric acid did not. The apparent Km of the transporter was 7.0 μM. After size fractionation on a sucrose density gradient, poly(A)+ RNA encoding for the MDCK taurine transporter was found in the fraction whose average size was 4.4 kilobases. | - |
dc.identifier.bibliographicCitation | JOURNAL OF BIOLOGICAL CHEMISTRY, v.266, no.15, pp.9605 - 9609 | - |
dc.identifier.issn | 0021-9258 | - |
dc.identifier.scopusid | 2-s2.0-0025914429 | - |
dc.identifier.uri | https://scholarworks.unist.ac.kr/handle/201301/4740 | - |
dc.identifier.url | http://www.scopus.com/inward/record.url?partnerID=HzOxMe3b&scp=0025914429 | - |
dc.identifier.wosid | A1991FM45900043 | - |
dc.language | 영어 | - |
dc.publisher | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC | - |
dc.title | EXPRESSION OF MADIN-DARBY CANINE KIDNEY-CELL NA+-DEPENDENT AND CL--DEPENDENT TAURINE TRANSPORTER IN XENOPUS-LAEVIS OOCYTES | - |
dc.type | Article | - |
dc.description.journalRegisteredClass | scopus | - |
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