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Author

Kwon, H. Moo
Inflammation and Kidney Disorder Lab
Research Interests
  • TonEBP, Obesity, Cancer, Chronic inflammatory diseases, Brain disorder, Kidney disorders, Genomic instability

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Cis- and trans-acting factors regulating transcription of the BGT1 gene in response to hypertonicity

Cited 103 times inthomson ciCited 107 times inthomson ci
Title
Cis- and trans-acting factors regulating transcription of the BGT1 gene in response to hypertonicity
Author
Miyakawa, HWoo, SKChen, CPDahl, SCHandler, JSKwon, H. Moo
Keywords
Betaine/γ-aminobutyric acid transporter; Compatible osmolytes; In vivo footprinting
Issue Date
199804
Publisher
AMER PHYSIOLOGICAL SOC
Citation
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY, v.274, no.4, pp.f753 - f761
Abstract
We have previously identified a tonicity-responsive enhancer (TonE) in the promoter region of the canine BGT1 gene. Tone mediates hypertonicity- induced stimulation of transcription. Here, we characterize Tone and Tone binding proteins (TonEBPs) to provide a biochemical basis for cloning of the TOnEBPs. Mutational analysis applied to both hypertonicity-induced stimulation of transcription and TonEBP binding reveals that Tone is 11 base pairs in length, with the consensus sequence of (C/T)GGAAnnn(C/T)n(C/T). Activity of the TonEBPs increases in response to hypertonicity with a time course similar to that of transcription of the BGT1 gene. Studies with inhibitors indicate that translation, but not transcription, is required for activation of the TonEBPs. Phosphorylation is required for the stimulation of transcription but not for activation of DNA binding by the TonEBPs. In vivo methylation by dimethyl sulfate reveals that the Tone site of the BGT1 gene is protected with a time course like that of activity of the TonEBPs and activation of transcription. Ultraviolet cross-linking indicates that the TonEBPs share a DNA binding subunit of 200 kDa.
URI
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ISSN
1931-857X
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